Site-Specific Nitrosoproteomic Identification of Endogenously S-Nitrosylated Proteins in Arabidopsis
Site-Specific Nitrosoproteomic Identification of Endogenously S-Nitrosylated Proteins in Arabidopsis
复制标题
拟南芥内源 S-亚硝基化蛋白的位点特异性亚硝基蛋白质组学鉴定
DOI：
10.1104/pp.15.00026
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发表时间：
2015-04-01
期刊：
影响因子：
7.4
通讯作者：
Zuo, Jianru
中科院分区：
文献类型：
--
作者：
Hu, Jiliang;Huang, Xiahe;Zuo, Jianru
一氧化氮（NO）调节植物的多种发育事件和胁迫反应。NO的主要生物活性物质是S-亚硝基谷胱甘肽（GSNO），其被GSNO还原酶（GSNOR）不可逆地降解。NO的主要生理作用是蛋白质S-亚硝基化，这是一种通过将NO分子共价连接到半胱氨酸巯基上的基于氧化还原的翻译后修饰机制。然而，很少有人知道S-亚硝基化调节信号的机制，部分原因是有限的S-亚硝基化蛋白被确定。在这项研究中，我们确定了1,195内源性S-亚硝基肽926蛋白质从拟南芥（拟南芥）的位点特异性亚硝基蛋白质组学的方法，这是迄今为止，最大的数据集的S-亚硝基蛋白质在所有生物体。这些肽的共有序列分析确定了几个基序，含有酸性，但不是碱性，氨基酸残基侧翼的S-亚硝基化半胱氨酸残基。这些S-亚硝基化蛋白质参与广泛的生物过程，并在叶绿素代谢、光合作用、碳水化合物代谢和胁迫反应中显著富集。因此，gsnor 1 -3突变体的叶绿素含量降低，光合特性改变，表明S-亚硝基化是其光合作用的重要调控机制。这些结果为植物S-亚硝基化调控信号的研究提供了宝贵的资源和新的线索。
Nitric oxide (NO) regulates multiple developmental events and stress responses in plants. A major biologically active species of NO is S-nitrosoglutathione (GSNO), which is irreversibly degraded by GSNO reductase (GSNOR). The major physiological effect of NO is protein S-nitrosylation, a redox-based posttranslational modification mechanism by covalently linking an NO molecule to a cysteine thiol. However, little is known about the mechanisms of S-nitrosylation-regulated signaling, partly due to limited S-nitrosylated proteins being identified. In this study, we identified 1,195 endogenously S-nitrosylated peptides in 926 proteins from the Arabidopsis (Arabidopsis thaliana) by a site-specific nitrosoproteomic approach, which, to date, is the largest data set of S-nitrosylated proteins among all organisms. Consensus sequence analysis of these peptides identified several motifs that contain acidic, but not basic, amino acid residues flanking the S-nitrosylated cysteine residues. These S-nitrosylated proteins are involved in a wide range of biological processes and are significantly enriched in chlorophyll metabolism, photosynthesis, carbohydrate metabolism, and stress responses. Consistently, the gsnor1-3 mutant shows the decreased chlorophyll content and altered photosynthetic properties, suggesting that S-nitrosylation is an important regulatory mechanism in these processes. These results have provided valuable resources and new clues to the studies on S-nitrosylation-regulated signaling in plants.