Phylogenetic study of two truffles, Tuber formosanum and Tuber furfuraceum, identified from Taiwan
Phylogenetic study of two truffles, Tuber formosanum and Tuber furfuraceum, identified from Taiwan
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DOI：
10.1111/j.1574-6968.2009.01571.x
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发表时间：
2009-05-01
影响因子：
2.1
通讯作者：
Shen, Wei-Chiang
中科院分区：
文献类型：
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作者：
Huang, Jing-Yang;Hu, Hung-Tao;Shen, Wei-Chiang
松露是最有价值的食用菌之一，在全世界范围内引起了广泛的研究兴趣。在台湾，已鉴定并报道了两种松露，即台湾块菌和糠秕块菌。尽管已经描述了这两种松露的形态特征，但缺乏分子鉴定导致难以牢固地确定它们与其他松露的相关性。在本研究中，我们利用内部转录间隔区（ITS）和β-微管蛋白基因序列来生成台湾毛毛虫和糠毛毛毛虫与其他分类学亲缘关系的系统发育关系。我们的分析根据 5.8S-ITS2/β-微管蛋白基因序列揭示了五/三个主要系统发育分支，并证实了它们的形态特征。台湾块茎与印度块茎 B 复合体高度相似，而糠秕块茎与惠东块茎最相似。根据分子钟，我们估计糠秕草和台湾狒狒与它们在中国大陆的近亲的分化时间分别为10.2 Ma和4.1 Ma。根据结果，我们推测在台湾发现的这两种块菌物种可能起源于中国某些块菌物种的共同祖先。但由于长时间的分化和地理上的隔离，它们已进化为台湾本土物种。
Truffles are one of the most valuable edible fungi and have drawn extensive research interests worldwide. In Taiwan, two species of truffle, Tuber formosanum and Tuber furfuraceum, have been identified and reported. Although the morphological features of these two truffles have been described, lack of molecular identification has led to difficulties with firmly establishing their relatedness to other truffles. In this study, we utilized the internal transcribed spacer (ITS) and beta-tubulin gene sequences to generate the phylogenetic relationship of T. formosanum and T. furfuraceum with other taxonomic relatives. Our analysis revealed five/three major phylogenetic clades according to the 5.8S-ITS2/beta-tubulin gene sequences and corroborated with their morphological characterization. Tuber formosanum highly resembles the Tuber indicum B complex, while T. furfuraceum is most similar to Tuber huidongense. Based on a molecular clock, we estimated that T. furfuraceum and T. formosanum would have diverged from their close relatives in mainland China between 10.2 and 4.1 Ma, respectively. Based on the results, we propose that these two Tuber species found in Taiwan might originate from the common ancestors with some truffle species in China. However, due to a long divergence time and geographical separation, they have evolved into indigenous species of Taiwan.