Cross-species analysis defines the conservation of anatomically segregated VMH neuron populations.
Cross-species analysis defines the conservation of anatomically segregated VMH neuron populations.
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DOI：
10.7554/elife.69065
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发表时间：
2021-05-21
期刊：
影响因子：
7.7
通讯作者：
Rupp AC
中科院分区：
文献类型：
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作者：
Affinati AH;Sabatini PV;True C;Tomlinson AJ;Kirigiti M;Lindsley SR;Li C;Olson DP;Kievit P;Myers MG;Rupp AC
下丘脑腹内侧核（VMH）控制着多种行为和生理功能，表明存在多种功能各异的下丘脑腹内侧核神经亚型。结合翻译核糖体亲和纯化与rna测序（TRAP-seq）数据和单核rna测序（snRNA-seq）数据，我们鉴定了24个小鼠VMH神经元簇。进一步的分析，包括来自猕猴组织的snRNA-seq数据，定义了一个更容易处理的VMH包装方案，包括6个主要的遗传和解剖分化的VMH神经元类别，具有良好的跨物种保护。除了两个主要的腹外侧类外，我们还鉴定了三种不同的背内侧VMH神经元。与先前提出的瘦素受体（Lepr）表达VMH神经元的独特作用一致，Lepr表达标志着单一的背内侧分类。我们还发现了一类谷氨酸能VMH神经元，它们位于VMH神经解剖学核心的前外侧结节区。这个保守的VMH神经元群图谱为VMH电路和功能的分析提供了一个无偏倚的起点。
The ventromedial hypothalamic nucleus (VMH) controls diverse behaviors and physiologic functions, suggesting the existence of multiple VMH neural subtypes with distinct functions. Combing translating ribosome affinity purification with RNA-sequencing (TRAP-seq) data with single-nucleus RNA-sequencing (snRNA-seq) data, we identified 24 mouse VMH neuron clusters. Further analysis, including snRNA-seq data from macaque tissue, defined a more tractable VMH parceling scheme consisting of six major genetically and anatomically differentiated VMH neuron classes with good cross-species conservation. In addition to two major ventrolateral classes, we identified three distinct classes of dorsomedial VMH neurons. Consistent with previously suggested unique roles for leptin receptor (Lepr)-expressing VMH neurons, Lepr expression marked a single dorsomedial class. We also identified a class of glutamatergic VMH neurons that resides in the tuberal region, anterolateral to the neuroanatomical core of the VMH. This atlas of conserved VMH neuron populations provides an unbiased starting point for the analysis of VMH circuitry and function.