3D printing of complex GelMA-based scaffolds with nanoclay
3D printing of complex GelMA-based scaffolds with nanoclay
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使用纳米粘土 3D 打印复杂的基于 GelMA 的支架
DOI：
10.1088/1758-5090/ab0cf6
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发表时间：
2019-07-01
期刊：
影响因子：
9
通讯作者：
He, Yong
中科院分区：
文献类型：
--
作者：
Gao, Qing;Niu, Xuefeng;He, Yong
可光交联型明胶甲基丙烯酸酯(GelMA)以其优异的生物学性能成为3D打印领域的一种极具吸引力的油墨。然而，受粘度低、交联时间长的限制，以挤出为基础的3D打印直接打印GelMA仍然是一个挑战。在这里，为了平衡可印刷性和生物相容性，特别设计了由GelMA和纳米粘土组成的生物材料墨水。利用纳米粘土的触变性，可以很容易地打印出形状逼真的复合支架。在本研究中，我们试图回答这种墨水的一些基本打印要求的问题，包括打印窗口、一般性能(孔隙率、机械强度等)和生物相容性。我们发现，GelMA/Nanoday墨水能够打印复杂的3D支架，如仿生耳和分支血管。此外，纳米粘土的加入改善了材料的孔隙率，提高了材料的机械强度，降低了材料的降解率，并保持了良好的生物相容性。因此，这种方法提供了一种简单的方法来打印具有良好形状保真度和生物学性能的复合支架，并可能为组织缺损的个性化治疗开辟新的潜在应用。
Photo-crosslinkable gelatin methacrylate (GelMA) has become an attractive ink in 3D printing due to its excellent biological performance. However, limited by low viscosity and long cross-linking time, it is still a challenge to directly print GelMA by extrusion-based 3D printing. Here, to balance the printability and biocompatibility, biomaterial ink composed of GelMA and nanoclay was specially designed. Using this ink, complex scaffolds with high shape fidelity can be easily printed based on the thixotropic property of nanoclay. In this study, we tried to answer some basic printing-required questions of this ink, including the printability window, general properties (porosity, mechanical strength, et al), and biocompatibility. We found that the GelMA/Nanoday ink enabled printing complex 3D scaffolds, such as a bionic ear and a branched vessel. Furthermore, the addition of nanoclay improved the porosity, increased the mechanical strength, reduced the degradation ratio, and maintained a good biocompatibility of the printed scaffolds. Therefore, this method offers an easy way to print complex scaffolds with good shape fidelity and biological performance, and it might open up new potential applications for the customized therapy of tissue defects.