The role of aquaporin-4 in the blood-brain barrier development and integrity: Studies in animal and cell culture models
The role of aquaporin-4 in the blood-brain barrier development and integrity: Studies in animal and cell culture models
复制标题
DOI：
10.1016/j.neuroscience.2004.07.055
复制
发表时间：
2004-01-01
期刊：
影响因子：
3.3
通讯作者：
Friger, A
中科院分区：
文献类型：
--
作者：
Nicchia, GP;Nico, B;Friger, A
水通道蛋白4（AQP4）是脑血管周围星形胶质细胞过程中表达的主要水通道。虽然AQP4在脑水肿中的作用已被广泛研究，但关于其在血脑屏障（BBB）中的功能作用的信息很少。这项工作的目的是整合以前和最近的数据，在血脑屏障形成和血脑屏障的完整性，使用几个实验模型的AQP4表达。鸡的视顶盖，一个完善的模型，血脑屏障的发展，和脂多糖对血脑屏障的完整性和血管周围的AQP4表达的影响的研究结果进行了分析和讨论。此外，BBB结构和水通道蛋白4的表达在杜氏肌营养不良症小鼠模型的数据进行了审查。特别地，已经将从mdX（3cv）小鼠获得的公开结果与从mdx小鼠获得的新数据一起进行了分析，在mdx小鼠中，包括DP71在内的所有抗肌萎缩蛋白同种型都强烈减少。最后，使用大鼠原代星形胶质细胞和脑毛细血管内皮细胞共培养物作为体外BBB模型，研究了内皮成分对AQP4细胞表达和分布的作用。(C)2004年IBRO。由爱思唯尔有限公司出版。保留所有权利。
Aquaporin-4 (AQP4) is the major water channel expressed in brain perivascular astrocyte processes. Although the role of AQP4 in brain edema has been extensively investigated, little information exists regarding its functional role at the blood-brain barrier (BBB). The purpose of this work is to integrate previous and recent data regarding AQP4 expression during BBB formation and depending on BBB integrity, using several experimental models. Results from studies on the chick optic tectum, a well-established model of BBB development, and the effect of lipopolysaccharide on the BBB integrity and on perivascular AQP4 expression have been analyzed and discussed. Moreover, data on the BBB structure and AQP4 expression in murine models of Duchenne muscular dystrophy are reviewed. In particular, published results obtained from mdX(3cv) mice have been analyzed together with new data obtained from mdx mice in which all the dystrophin isoforms including DP71 are strongly reduced. Finally, the role of the endothelial component on AQP4 cellular expression and distribution has been investigated using rat primary astrocytes and brain capillary endothelial cell co-cultures as an in vitro model of BBB. (C) 2004 IBRO. Published by Elsevier Ltd. All rights reserved.