QUANTITATIVE MONITORING OF SOLID-PHASE PEPTIDE-SYNTHESIS BY THE NINHYDRIN REACTION
QUANTITATIVE MONITORING OF SOLID-PHASE PEPTIDE-SYNTHESIS BY THE NINHYDRIN REACTION
复制标题
DOI：
10.1016/0003-2697(81)90704-1
复制
发表时间：
1981-01-01
影响因子：
2.9
通讯作者：
MERRIFIELD, RB
中科院分区：
文献类型：
--
作者：
SARIN, VK;KENT, SBH;MERRIFIELD, RB
建立了固相多肽合成过程中游离氨基的快速、灵敏的定量测定方法。该技术包括在严格控制的条件下将游离胺与吲哚发生反应，并在570 nm的波长下测定生成的生色团。吲哚与伯胺反应的蓝色生色团全部存在于溶液中，聚苯乙烯载体上的珠子是无色的。提出了用前人的方法形成蓝珠的离子交换机理。该技术可用于测量树脂上的肽链总数，特别是用于监测偶联反应的进展。可以确定多肽键的形成何时完成99%。
A rapid and sensitive method for the quantitative determination of free amino groups during solid-phase peptide synthesis was developed. The technique involves the reaction of the free amine with ninhydrin under carefully controlled conditions and the determination of the resulting chromophore in solution at 570 nm. All of the blue chromophore of the ninhydrin reaction with primary amines is found in solution and the beads of the polystyrene support are colorless. An ion-exchange mechanism for the formation of blue beads by previous methods is proposed. The technique is useful for measuring the total number of peptide chains on the resin and, especially, for monitoring the progress of the coupling reaction. It is possible to determine when the formation of a peptide bond is > 99% complete.