Growth response of Populus trichocarpa to inoculation by the ectomycorrhizal fungus Laccaria laccata in a pot and a field experiment
Growth response of Populus trichocarpa to inoculation by the ectomycorrhizal fungus Laccaria laccata in a pot and a field experiment
复制标题
DOI：
10.1016/s0378-1127(01)00534-5
复制
发表时间：
2002-06-28
影响因子：
3.7
通讯作者：
Makeschin, F
中科院分区：
文献类型：
--
作者：
Baum, C;Stetter, U;Makeschin, F
毛果毛白杨的生长反应。在盆栽和田间试验中，研究了外生菌根(ECM)真菌Laccaria laaccata对Muhle Larsen接种的影响。盆栽试验取自德国下萨克森州Wildeshausen的未灭菌土壤。田间试验是在德国萨克森州Leubnitz的Stagnic Glaysols上的一个再造林人工林中进行的。由于缺乏寄主植物，这两种可耕作土壤的本土ECM接种量都很低。本研究旨在测定(1)盆栽和田间接种条件下，ECM在杨树无性系上的定植情况；(2)接种对植物生长参数的影响。盆栽试验中，对照植株7%的细根和接种对照植株的18%的细根在一个生长季节后被ECM真菌侵染。在田间试验中，在第一个生长季节后，对照小区有6%的细根被ECM真菌侵染，接种小区有20%的细根被ECM真菌侵染。在第二个生长季之后，分别有50%和53%的植物被定植。在盆栽和田间试验中，接种L漆树可显著增加杨树无性系在第一个生长季后的地上部长度和叶片钾素含量。在第二个生长季节，只有叶片中的钾浓度比未接种的对照植株进一步增加。接种后土壤中VAM孢子密度和叶片中氮、镁、钙浓度显著降低。然而，在第二个生长季之后，接种和未接种的杨树插条的ECM定殖率和新梢长度不再有显著差异。结果表明，接种能有效提高毛果青霉在原耕地土壤上的ECM定殖率和生长速率。这可以提高插条对土传病原菌的抗性，以及它们对养分和杂草空间的竞争力。(C)2002 Elsevier Science B.V.保留所有权利。
The growth response of Populus trichocarpa cv. Muhle Larsen to inoculation by the ectomycorrhizal (ECM) fungus Laccaria laccata was investigated in a pot and a field experiment on arable soils. Non-sterilized soil from a Cambisol in Wildeshausen (Lower Saxonia, Germany) was taken for the pot experiment. The field experiment was established at a reforestation plantation on Stagnic Gleysols in Leubnitz (Saxonia, Germany). Both arable soils were assumed to be low in indigenous ECM inoculum caused by the lack of host plants. The study was designed to determine (1) the ECM colonization on the poplar clone with or without inoculation in the pot and in the field experiment and (2) the effects of inoculation on plant growth parameters. In the pot experiment, 7% of fine roots of non-inoculated control plants and 18% of inoculated test plants were colonized with ECM fungi after one growing season. In the field experiment, 6% of fine roots were colonized with ECM fungi at the control plot and 20% at the inoculated plot after the first growing season. After the second growing season 50 or 53% were colonized, respectively. The inoculation by L laccata caused significantly increased shoot lengths and leaf potassium concentrations of the poplar clone in the pot and field experiment after the first growing season. In the second growing season, only the leaf potassium concentrations were further increased compared to the non-inoculated control plants. The density of VAM spores in the soil and the leaf nitrogen, magnesium and calcium concentrations were significantly reduced after inoculation. However, after the second growing season there were no longer were significant differences in the ECM colonization and shoot lengths of inoculated or non-inoculated poplar cuttings. The results indicated that inoculation can be successfully used to increase ECM colonization and growth rates of P. trichocarpa on former arable soils in the first growth period. This could increase the resistance of the cuttings to soil-borne pathogens and their competitiveness for nutrients and space against weeds. (C) 2002 Elsevier Science B.V. All rights reserved.