Zebrafish phosvitin-derived peptide Pt5 inhibits melanogenesis via cAMP pathway
Zebrafish phosvitin-derived peptide Pt5 inhibits melanogenesis via cAMP pathway
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斑马鱼卵黄高磷蛋白衍生肽 Pt5 通过 cAMP 途径抑制黑素生成
DOI：
10.1007/s10695-016-0306-3
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发表时间：
2017-04-01
影响因子：
2.9
通讯作者：
Zhang, Shicui
中科院分区：
文献类型：
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作者：
Liu, Yuan-Yuan;Su, Xiu-Rong;Zhang, Shicui
斑马鱼卵黄高磷蛋白衍生肽Pt 5由卵黄高磷蛋白C末端55个残基组成，已被证明具有与卵黄高磷蛋白相当的抗菌免疫调节活性。在这里，我们清楚地表明，Pt 5有能力抑制酪氨酸酶（TYR）的活性和黑色素的生物合成，这种抑制是独立的细胞增殖和细胞毒性作用。用异硫氰酸荧光素（FITC）标记的Pt 5与B16 F10黑色素瘤细胞孵育，发现Pt 5定位于细胞的细胞质中。此外，Pt 5抑制B16 F10黑色素瘤细胞中TYR、酪氨酸酶相关蛋白-1（TRP-1）、酪氨酸酶相关蛋白-2（TRP-2）和小眼相关转录因子（MITF）的表达，并降低细胞内环磷酸腺苷（cAMP）浓度，但不影响细胞内pERK 1/2和β-catenin的含量，提示Pt 5通过cAMP信号通路而不是Wnt和MAPK通路调节黑色素的生物合成。总之，这些数据表明，Pt 5有可能作为一种黑素生成抑制剂在医疗和化妆品行业，一个新的作用以往报道。
Zebrafish phosvitin-derived peptide Pt5, consisting of the C-terminal 55 residues of phosvitin, has been shown to have an antimicrobial-immunomodulatory activity comparable to phosvitin. Here, we showed clearly that Pt5 had the capacity to inhibit tyrosinase (TYR) activity and melanin biosynthesis, and this inhibition was independent of cell proliferation and cytotoxic effects. Incubation of fluorescein isothiocyanate (FITC)-labeled Pt5 with B16F10 melanoma cells revealed that Pt5 was localized in the cytoplasm of the cells. In addition, Pt5 inhibited the expression of TYR, tyrosinase-related protein-1 (TRP-1), tyrosinase-related protein-2 (TRP-2), and microphthalmia-associated transcription factor (MITF) in B16F10 melanoma cells and reduced the intracellular cyclic adenosine monophosphate (cAMP) concentration in the cells, but it did not affect the cellular contents of pERK1/2 and β-catenin, suggesting that Pt5 regulates melanin biosynthesis via cAMP signaling pathway rather than Wnt and MAPK pathways. Collectively, these data indicate that Pt5 has the potential to be used as a melanogenesis inhibitor in medical and cosmetic industry, a novel role ever reported.