FLOW BEHAVIOR OF ERYTHROCYTES .2. PARTICLE MOTIONS IN CONCENTRATED SUSPENSIONS OF GHOST CELLS
FLOW BEHAVIOR OF ERYTHROCYTES .2. PARTICLE MOTIONS IN CONCENTRATED SUSPENSIONS OF GHOST CELLS
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DOI：
10.1016/0021-9797(79)90248-0
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发表时间：
1979-01-01
影响因子：
9.9
通讯作者：
MARLOW, JC
中科院分区：
文献类型：
--
作者：
GOLDSMITH, HL;MARLOW, JC
在显微镜下，在红细胞血影（用作全血的模型）的透明悬浮液中，通过在雷诺数为10-3-0.3和体积浓度（c）为0.10-0.93的条件下跟踪流经半径Ro为32-80 μ m的管中的颗粒，研究了个体人红细胞（RBC）的详细运动。RBC速度分布在c > 0.2处从抛物线变钝，在管的核心中具有半径Rc的活塞流区域，其中速度μ M可测量地恒定。Rc随c的增大而增大，随流量Q和Ro的增大而减小。在c > 0.4时，RBC从静止的双凹形状连续变形，并且它们的长轴在20 °内对齐。of the flow流.即使在c = 0.93时，谷胱甘肽硬化红细胞（HBC）也继续以不同的角速度旋转。RBC的路径表现出不规则的径向位移，其幅度和频率在给定的c下随着剪切速率的增加而增加，并且在给定的管中在0.2 < c < 0.4时最大，并且颗粒在径向位置0.4 < R/Ro < 0.8。在存在0.01 μ M/Rc量级的剪切速率的活塞流区域中也发生明显的径向位移。同时测定了红细胞在试管中面的分布。在高Q和c < 0.43时，红细胞明显向外周移动，这可能是由于红细胞和血影之间的径向迁移速率不同，这是由于它们的密度不同。
The detailed motions of individual human red blood cells (RBC) were studied under the microscope in transparent suspensions of red cell ghosts (serving as a model for whole blood) by tracking the particles in flow through tubes having radii Ro of 32-80 .mu.m at Reynolds numbers from 10-3-0.3 and volume concentrations (c) from 0.10-0.93. The RBC velocity distributions were blunted from parabolic at c > 0.2 with a region of plug flow of radius Rc in the core of the tube where the velocities .mu.M were measurably constant. Rc increased with increasing c, and decreased with increasing flow rate Q and Ro. At c > 0.4, the RBC were continuously deformed from the resting biconcave shape and their major axes became aligned within 20.degree. of the flow. Gluteraldehyde-hardened red cells (HBC) continued to rotate with varying angular velocities even at c = 0.93. The paths of the RBC exhibited erratic radial displacements whose magnitude and frequency at a given c increased with increasing shear rate, and in a given tube was greatest at 0.2 < c < 0.4 and with particles at radial positions 0.4 < R/Ro < 0.8. Appreciable radial displacements also occurred in the plug flow region where shear rates of the order 0.01 .mu.M/Rc existed. The distributions of RBC across the median plane of the tube were also measured. At high Q and c < 0.43 there was a pronounced movement of RBC to the periphery, perhaps due to differences in the rates of radial migration between RBC and ghosts stemming from differences in their densities.