Distinction between alpha-fetoprotein and intracellular estrogen receptors: evidence against the presence of estradiol receptors in rat bone.
Distinction between alpha-fetoprotein and intracellular estrogen receptors: evidence against the presence of estradiol receptors in rat bone.
复制标题
甲胎蛋白和细胞内雌激素受体之间的区别：反对大鼠骨中存在雌二醇受体的证据。
作者：
T. Chen;D. Feldman
骨质疏松症与绝经后状态的关联以及雌激素替代疗法可能有效治疗骨质疏松过程的信念，使人们对雌激素对骨的作用产生了相当大的兴趣。本研究的目的是确定是否可以在大鼠骨中证明雌二醇的特异性受体位点，从而为雌激素在骨细胞水平的直接作用提供依据。细胞质提取物从各种骨孵育与[3H]雌二醇和结合受体的葡聚糖凝胶色谱法进行了测量。培养的骨细胞从胎儿颅骨和细胞质从成年卵巢切除女性未能表现出雌激素受体，虽然糖皮质激素受体可以证明如前所述。混合实验表明，受体的缺乏是由于骨胞质溶胶中的其他因素的干扰或通过连接的受体骨羟基磷灰石。检查刚从围产期分离出来的骨头
The association of osteoporosis with the post-menopausal state and the belief that estrogen replacement may be efficacious in treating the osteoporotic process has created considerable interest in the action of estrogens on bone. The purpose of the present study was to ascertain whether specific receptor sites for estradiol could be demonstrated in rat bone, thus providing a basis for the direct action of estrogen at the bone cell level. Cytoplasmic extracts from a variety of bones were incubated with [3H]estradiol and bound receptors were measured by Sephadex chromatography. Cultured bone cells from fetal calvaria and cytosol from adult ovariectomized females failed to exhibit estrogen receptors, although glucocorticoid receptors could be demonstrated as previously described. Mixing experiments indicated that the absence of receptors was not due to interference by the other factors in bone cytosol or by attachment of receptors to bone hydroxylapatite. Examination of bones freshly isolated from perinatal ...