Orally administered [14C]DPA and [14C]DHA are metabolised differently to [14C]EPA in rats
Orally administered [14C]DPA and [14C]DHA are metabolised differently to [14C]EPA in rats
复制标题
DOI：
10.1017/s0007114512001419
复制
发表时间：
2013-02-14
影响因子：
3.6
通讯作者：
Sinclair, Andrew J.
中科院分区：
文献类型：
--
作者：
Kaur, Gunveen;Molero, Juan C.;Sinclair, Andrew J.
先前的研究表明，与SFA，MUFA和C18 PUFA相比，C20 PUFA在全身研究中氧化为CO2的程度明显较低。本研究确定了三种长链多不饱和脂肪酸，即20：5 n-3 EPA，22：5 n-3二十二碳五烯酸（DPA）和22：6 n-3 DHA分解代谢为CO2或相反地并入组织脂质的程度。大鼠单次经口给予2.5 μ Ci [1-C-14] DPA、[1-C-14] EPA、[1-C-14] DHA或[1-C-14]油酸（18：1 n-9; OA），并置于代谢室中6 h，在代谢室中捕获呼出的（CO2）-C-14并计数放射性。24小时后对大鼠实施安乐死，取出组织用于分析组织脂质中的放射性。结果表明，DPA和DHA分解为CO2的量显著低于EPA和OA（P < 0.05）。磷脂（PL）馏分是最标记的所有三个n-3 PUFA相比，OA在所有组织中，并有C20和C22 n-3 PUFA的PL馏分中的标签的比例没有差异。在骨骼肌和心脏中，DHA和DPA组比EPA组显示出显著更多的标记。在脑和心脏组织中，与C20 n-3 PUFA组相比，C22 n-3 PUFA组的胆固醇部分中的标记显著较少。与EPA相比，DHA和DPA更高地掺入心脏和骨骼肌中，表明这些C22 n-3 PUFA可能在这些组织中起重要作用。
Previous studies have revealed that C20 PUFA are significantly less oxidised to CO2 in whole-body studies compared with SFA, MUFA and C18 PUFA. The present study determined the extent to which three long-chain PUFA, namely 20 : 5n-3 EPA, 22 : 5n-3 docosapentaenoic acid (DPA) and 22 : 6n-3 DHA, were catabolised to CO2 or, conversely, incorporated into tissue lipids. Rats were administered a single oral dose of 2.5 mu Ci [1-C-14] DPA, [1-C-14] EPA, [1-C-14] DHA or [1-C-14] oleic acid (18 : 1n-9; OA), and were placed in a metabolism chamber for 6 h where exhaled (CO2)-C-14 was trapped and counted for radioactivity. Rats were euthanised after 24 h and tissues were removed for analysis of radioactivity in tissue lipids. The results showed that DPA and DHA were catabolised to CO2 significantly less compared with EPA and OA (P < 0.05). The phospholipid (PL) fraction was the most labelled for all three n-3 PUFA compared with OA in all tissues, and there was no difference between C20 and C22 n-3 PUFA in the proportion of label in the PL fraction. The DHA and DPA groups showed significantly more label than the EPA group in both skeletal muscle and heart. In the brain and heart tissue, there was significantly less label in the cholesterol fraction from the C22 n-3 PUFA group compared with the C20 n-3 PUFA group. The higher incorporation of DHA and DPA into the heart and skeletal muscle, compared with EPA, suggests that these C22 n-3 PUFA might play an important role in these tissues.