Induction of interleukin (IL)-8 gene expression by respiratory syncytial virus involves activation of nuclear factor (NF)-kappa B and NF-IL-6
Induction of interleukin (IL)-8 gene expression by respiratory syncytial virus involves activation of nuclear factor (NF)-kappa B and NF-IL-6
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DOI：
10.1093/infdis/174.2.262
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发表时间：
1996-08-01
影响因子：
6.4
通讯作者：
Hunninghake, GW
中科院分区：
文献类型：
--
作者：
Mastronarde, JG;He, B;Hunninghake, GW
呼吸道合胞病毒（RSV）优先感染呼吸道上皮，是幼儿下呼吸道感染的重要原因。RSV诱导气道上皮细胞产生白细胞介素（IL）-8;然而，这种诱导的机制尚不清楚。为了确定RSV诱导IL-8基因活化的机制，用含有IL-8基因5 '侧翼区连续缺失的质粒转染A549上皮细胞，然后暴露于RSV 24小时。观察到转录因子核因子（NF）-κ B和NF-IL-6的结合位点的正协同效应。任一区域的突变减弱启动子对RSV感染的响应性。RSV还增加NF-κ B和NF-IL-6转录因子的活化。这些数据表明，RSV可能部分通过激活转录因子NF-κ B和NF-IL-6来增加气道上皮细胞中IL-8的产生。
Respiratory syncytial virus (RSV) preferentially infects respiratory epithelium and is an important cause of lower respiratory tract infections in young children. RSV induces the production of interleukin (IL)-8 in airway epithelial cells; however, the mechanism of this induction is not known. To define the mechanism by which RSV induces IL-8 gene activation, A549 epithelial cells were transfected with plasmids containing serial deletions of the 5'-flanking region of the IL-8 gene and then exposed to RSV for 24 h. A positive cooperative effect of the binding sites for the transcription factors, nuclear factor (NF)-kappa B and NF-IL-6, was observed. Mutations in either region abates responsiveness of the promoter to RSV infection. RSV also increases activation of the NF-kappa B and NF-IL-6 transcription factors. These data suggest that RSV may increase IL-8 production in airway epithelium partly via activation of the transcription factors NF-kappa B and NF-IL-6.