THE DISTRIBUTION OF TAU AND HMW MICROTUBULE-ASSOCIATED PROTEINS IN DIFFERENT CELL-TYPES
THE DISTRIBUTION OF TAU AND HMW MICROTUBULE-ASSOCIATED PROTEINS IN DIFFERENT CELL-TYPES
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DOI：
10.1016/0014-4827(80)90439-5
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发表时间：
1980-01-01
影响因子：
3.7
通讯作者：
KALNINS, VI
中科院分区：
文献类型：
--
作者：
CONNOLLY, JA;KALNINS, VI
为了研究tau蛋白和高分子量（HMW）微管相关蛋白（MAPs）在体内的分布以及它们与微管的关系，我们用针对这两种蛋白的抗血清通过免疫荧光法对多种禽类和哺乳类细胞类型进行了检测。到目前为止，在所检测的所有哺乳类细胞类型中，抗HMW血清都能使细胞质微管着色。抗tau血清在大鼠神经胶质细胞或猪肾细胞中不能使细胞质微管着色。在大鼠神经元、小鼠和猪的成纤维细胞以及小鼠神经母细胞瘤N2A - A4和N - 115细胞中，两种血清对微管的着色情况相似。抗HMW血清不能使原纤毛或分离的猪和鸡气管上皮细胞的纤毛着色，而抗tau血清却能使这些纤毛微管着色。某些类型的微管可能只与tau蛋白或HMW蛋白相关联，而其他类型的微管可能与tau蛋白和HMW蛋白都相关联。对于禽类细胞，抗HMW血清在所检测的3种细胞类型中都不能使微管着色，而抗tau血清能使其中2种细胞类型的微管着色。双向扩散试验表明，抗猪tau血清能使猪脑tau蛋白和从鸡脑分离出的tau蛋白都发生沉淀，而抗HMW血清只能使猪脑的HMW蛋白发生沉淀，而不能使鸡脑的HMW蛋白沉淀。显然，tau蛋白在禽类和哺乳类细胞中在抗原性上相似，而来自这两种来源的HMW蛋白在抗原性上是不同的。
To investigate the distribution of the tau and HMW [high molecular weight] microtubule-associated proteins (MAPS) and their relationship to microtubules in vivo, a wide variety of avian and mammalian cell types were examined by immunofluorescence with antisera to these 2 proteins. Anti-HMW serum stains cytoplasmic microtubules in all mammalian cell types so far examined. Anti-tau serum did not stain cytoplasmic microtubules in rat glial cells or in pig kidney cells. In rat neurons, mouse and pig fibroblasts and mouse neuroblastoma N2A-A4 and N-115 cells, the staining of microtubules with both sera was similar. Anti-HMW serum did not stain primary cilia or cilia on isolated pig and chicken tracheal epithelial cells, whereas anti-tau serum did stain these ciliary microtubules. Some types of microtubules may be associated with only the tau or the HMW protein, whereas others may be associated with both tau and HMW protein. With respect to avian cells, anti-HMW serum did not stain microtubules in any of the 3 cell types examined, whereas the anti-tau serum stained them in 2 cell types. Double diffusion tests indicated that anti-pig tau serum will precipitate both pig brain tau and tau protein isolated from chick brain, whereas anti-HMW serum will precipitate only pig brain and not chick brain HMW protein. Tau protein apparently is antigenically similar in both avian and mammalian cells, whereas the HMW protein from these 2 sources is antigenically distinct.