Purification of innate immunostimulant from green tea using a silkworm muscle contraction assay
Purification of innate immunostimulant from green tea using a silkworm muscle contraction assay
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DOI：
10.5582/ddt.v5.1.18
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发表时间：
2011-01-01
影响因子：
3.1
通讯作者：
Sekimizu, Kazuhisa
中科院分区：
文献类型：
--
作者：
Dhital, Saphala;Hamamoto, Hiroshi;Sekimizu, Kazuhisa
通过测定绿茶热水提取物对家蚕幼虫的免疫刺激活性，纯化了绿茶多糖。核磁共振和酸水解物的化学分析表明，纯化的物质具有含有聚半乳糖醛酸和甲酯残基的主链。用β -葡聚糖酶处理后，纯化样品的肌肉收缩活性减弱，这表明β -葡聚糖结构（可能是分支形式）是其活性所必需的。纯化后的部分刺激小鼠腹腔巨噬细胞产生白细胞介素-6。这些结果表明，测定家蚕幼虫的免疫刺激对评价各种来源的先天免疫刺激剂是有用的。
A polysaccharide was purified from a hot water extract of green tea leaves by measuring the immunostimulatory activity in silkworm larvae. Nuclear magnetic resonance and chemical analysis of acid hydrolysates revealed that the purified substance possessed a backbone containing polygalacturonic acids with methyl ester residues. Treatment with beta-glucanase attenuated the muscle contraction activity of the purified sample, suggesting that the beta-glucan structure, probably as a branched form, was required for its activity. The purified fraction stimulated the production of interleukin-6 by mouse peritoneal macrophages. These results suggest that measuring immunostimulation in silkworm larvae is useful for evaluating innate immunostimulants from various sources.