Jet ring cell: a tool for flow injection spectroscopy and microscopy on a renewable solid support.

Jet ring cell: a tool for flow injection spectroscopy and microscopy on a renewable solid support.
复制标题

射流环池:一种在可再生固体支持物上进行流动注射光谱和显微镜检查的工具。

DOI:
10.1021/ac00072a006
复制
发表时间:
1993
影响因子:
7.4
通讯作者:
Scudder,KM
Scudder,KM
中科院分区:
化学1区
文献类型:
--
作者:
Ruzicka,J;Pollema,CH;Scudder,KM

文献摘要

被引文献

相似文献

本文介绍了一种用于悬浮液光谱测量的新型流动池--喷射环池。该池利用通过狭窄环形间隙的径向流动将悬浮颗粒保留在检测区域内。这个环构成了一个检测体积的明确界定的区域,其中捕获的颗粒可以立即删除的意愿。因此,颗粒床形成了一个可再生的表面,可以使用显微镜或光纤通过反射、荧光或微发光进行探测。该装置应证明是有用的细胞的显微镜研究,自动化免疫测定,并在原位光谱检测的吸附剂上的分析物的预浓缩。结合光纤检测系统,喷射环单元成为可再生化学传感器系统的一个组成部分。
A new flow cell design for spectroscopic measure-ments of suspensions, the jet ring cell, is intro-duced. This cell exploits radial flow through a narrow ring-shaped gap to retain suspended par-ticles within the detection region. This ring constitutes a detection volume of well-defined area from which thetrapped particles can be instantaneously removed at will. The bed of particles thus forms a renewable surface, which can be probed by reflectance, fluorescence, or chemilu-minescence using a microscope or optical fiber. This device should prove useful for microscopic study of cells, for automated immunoassays, and for preconcentration of analytes on sorbents with in situ spectroscopic detection. In conjunction with a fiber optic detection system, the jet ring cell becomes a component of a renewable chemical sensor system.