Activity-based therapies to promote forelimb use after a cervical spinal cord injury.
Activity-based therapies to promote forelimb use after a cervical spinal cord injury.
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DOI：
10.1089/neu.2008-0592
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发表时间：
2009-10
影响因子：
4.2
通讯作者：
Bregman BS
中科院分区：
文献类型：
--
作者：
Dai H;MacArthur L;McAtee M;Hockenbury N;Tidwell JL;McHugh B;Mansfield K;Finn T;Hamers FP;Bregman BS
人们对改善脊髓损伤后前肢功能的策略非常感兴趣。我们研究了丰富的住房和熟练训练相结合对成年大鼠颈脊髓损伤后熟练和自动前肢功能恢复的影响。所有的动物都接受了熟练的伸展、网格行走和地面运动的训练。一些人接受了C4-5的颈椎过半横断损伤，打断了右侧脊髓和背柱，并被单独安置在标准住房或经过日常训练的丰富环境中。一部分动物接受了罗利普兰治疗，以促进神经元的可塑性。每周对动物进行为期4周的测试，以测量触觉、网格行走中的错误、运动和垂直探索。将生物素化的葡聚糖胺注射到皮质，以标记皮质脊髓束。与标准住房相比，丰富的环境/日常培训显著增加了左房的数量和成功率。动物在网格行走中犯的错误也更少，这是一种衡量协调前肢功能的指标。然而，在垂直探索或移动过程中，前肢的使用没有明显的改善。同样，罗利普兰没有改善任何测试的行为。强化壳聚糖和罗力普兰均可增加病变吻侧皮质脊髓束的可塑性。这些研究表明，颈髓损伤后的熟练训练可以改善熟练的前肢使用(伸展)和协调肢体功能(网格行走)的恢复，但不能改善自动前肢功能(运动和垂直探索)。这些研究表明，脊髓损伤后恢复前肢功能需要对下行通路和节段性通路分别采取不同的策略。
Significant interest exists in strategies for improving forelimb function following spinal cord injury. We investigated the effect of enriched housing combined with skilled training on the recovery of skilled and automatic forelimb function after a cervical spinal cord injury in adult rats. All animals were pretrained in skilled reaching, gridwalk crossing, and overground locomotion. Some received a cervical over-hemisection lesion at C4-5, interrupting the right side of the spinal cord and dorsal columns bilaterally, and were housed in standard housing alone or enriched environments with daily training. A subset of animals received rolipram to promote neuronal plasticity. Animals were tested weekly for 4 weeks to measure reaching, errors on the gridwalk, locomotion, and vertical exploration. Biotinylated dextran amine was injected into the cortex to label the corticospinal tract. Enriched environments/daily training significantly increased the number and success of left reaches compared to standard housing. Animals also made fewer errors on the gridwalk, a measure of coordinated forelimb function. However, there were no significant improvements in forelimb use during vertical exploration or locomotion. Likewise, rolipram did not improve any of the behaviors tested. Both enriched housing and rolipram increased plasticity of the corticospinal tract rostral to the lesion. These studies indicate that skilled training after a cervical spinal cord injury improves recovery of skilled forelimb use (reaching) and coordinated limb function (gridwalk) but does not improve automatic forelimb function (locomotion and vertical exploration). These studies suggest that rehabilitating forelimb function after spinal cord injury will require separate strategies for descending and segmental pathways.