A SENSITIVE REPORTER CELL-LINE FOR HIV-1 TAT ACTIVITY, HIV-1 INHIBITORS, AND T-CELL ACTIVATION EFFECTS
A SENSITIVE REPORTER CELL-LINE FOR HIV-1 TAT ACTIVITY, HIV-1 INHIBITORS, AND T-CELL ACTIVATION EFFECTS
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DOI：
10.1089/aid.1994.10.295
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发表时间：
1994-03-01
影响因子：
1.5
通讯作者：
BELMONT, JW
中科院分区：
文献类型：
--
作者：
AGUILARCORDOVA, E;CHINEN, J;BELMONT, JW
描述了一种用于HIV-1 TAT功能和T细胞活化定量检测的细胞系的制备和特性。1G5是由HLV-1长末端重复序列(HIV-LTR)驱动的荧光素酶基因稳定转染的Jurkat T细胞克隆细胞系。选择1G5克隆是因为基础荧光素酶活性低，对HIV感染的敏感性，以及对TAT和T细胞激活信号的高反应性。观察到TAT表达载体或HIV感染后，荧光素酶活性增加了10到1000倍。经T细胞有丝分裂原刺激后，检测到同等水平的表达。1G5的特性使其成为研究HIV感染、HIV调节剂和其他T细胞或HIV激活因子以及筛选潜在的抗HIV治疗药物的有价值的试剂。
The production and characterization of a cell line for quantitative HIV-1 Tat function and T cell activation assays is described. 1G5 is a clonal cell line derived from Jurkat T cells stably transfected with a luciferase gene driven by an HLV-1 long terminal repeat (HIV-LTR). The 1G5 clone was selected for low basal luciferase activity, susceptibility to HIV infection, and high responsiveness to Tat and T cell activation signals. A 10 to 1000-fold increase in luciferase activity after transfection or infection with tat-expressing vectors or HIV was observed. Equivalent levels of expression were detected after stimulation with T cell mitogens. The characteristics of 1G5 make it a valuable reagent for studies of HIV infection, HIV regulatory agents, and other T cell or HIV-activating factors, and for screening potential anti-HIV therapeutic agents.