Molecular Analysis of Chinese Celastrus and Tripterygium and Implications in Medicinal and Pharmacological Studies.
Molecular Analysis of Chinese Celastrus and Tripterygium and Implications in Medicinal and Pharmacological Studies.
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中国南蛇藤和雷公藤的分子分析及其在药物和药理学研究中的意义
DOI：
10.1371/journal.pone.0169973
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Zhang ZX
中科院分区：
文献类型：
--
作者：
Mu XY;Zhao LC;Zhang ZX
南蛇藤属和雷公藤属植物是中药中常用的两种植物，除了在自身免疫性疾病中的应用外，由于其抗肿瘤促进和神经保护活性而引起了人们的广泛关注。然而，它们之间的系统关系和物种之间的不清楚，这可能会影响它们的进一步药用。本研究对中国南蛇藤属和雷公藤属植物的叶绿体和核标记进行了分子标记分析，揭示了其属内和属间的亲缘关系。结果表明，雷公藤属植物在南蛇藤属植物中构成了一个天然的单系分支，具有较强的支持价值。在亚属分类上，果型和种子型优于花序型。中国南蛇藤属（Celastrus）植物分为三组：Sect.Sempervirentes（Maxim.）Cheng CY & Kao TC，Sect.Lunatus XY Mu & Zhang ZX，sect.nov.，和椭圆组Sect.Ellipticus XY Mu & ZX Zhang，sect.nov.的系统发育数据与文献报道的化学成分一致。由于南蛇藤属植物的亲缘关系较近，故推荐几种万年青南蛇藤属植物进行化学和药理研究。本研究结果也为南蛇藤和雷公藤的分子鉴定提供了参考。
Celastrus and Tripterygium species, which are used in traditional Chinese medicine, have attracted much attention due to their anti-tumor promoting and neuroprotective activities, in addition to their applications in autoimmune disorders. However, systematic relationships between them and among species are unclear, and it may disturb their further medicinal utilization. In the present study, the molecular analysis of combined chloroplast and nuclear markers of all Chinese Celastrus and Tripterygium was performed, and clear inter- and intra-genus relationships were presented. The result suggests that Tripterygium constitute a natural monophyletic clade within Celastrus with strong support value. Fruit and seed type are better than inflorescence in subgeneric classification. Chinese Celastrus are classified for three sections: Sect. Sempervirentes (Maxim.) CY Cheng & TC Kao, Sect. Lunatus XY Mu & ZX Zhang, sect. nov., and Sect. Ellipticus XY Mu & ZX Zhang, sect. nov. The phylogenetic data was consistent with their chemical components reported previously. Owing to the close relationship, several evergreen Celastrus species are recommended for chemical and pharmacological studies. Our results also provide reference for molecular identification of Chinese Celastrus and Tripterygium.