Human skeletal growth factor: characterization of the mitogenic effect on bone cells in vitro.
Human skeletal growth factor: characterization of the mitogenic effect on bone cells in vitro.
复制标题
人骨骼生长因子：体外对骨细胞有丝分裂作用的表征。
DOI：
10.1021/bi00257a038
复制
发表时间：
1982
期刊：
影响因子：
2.9
通讯作者：
Baylink,DJ
中科院分区：
文献类型：
--
作者：
Farley,JR;Masuda,T;Wergedal,JE;Baylink,DJ
John R.法利，T.增田，* J.E. Wergedal和大卫J. Baylink* 摘要：前面的论文[法利，J.R.，& Baylink，D. J.（1982）Biochemistry（在此问题上的前一篇论文）]详细描述了从人骨基质中纯化一种有效的生长因子。纯化的蛋白质（Mr 83000）被证明，在体外，既增加了鸡胚颅骨细胞的增殖率和鸡胚骨的生长率。我们现在报告，这种人骨骼生长因子对鸡颅骨细胞增殖在体外的影响是饱和的，延长，和诱导。该效应是饱和的，因为剂量/反应研究显示在1 mL体积中的Kmipp为12.5 nM/103个细胞。的绝对值
John R. Farley, T. Masuda,* J. E. Wergedal, and David J. Baylink* abstract: The preceding paper [Farley, J. R., & Baylink, D. J.(1982) Biochemistry (preceding paper in this issue)] detailed the purification of a potent growth factor from human bone matrix. The purified protein (Mr 83000) was shown to increase, in vitro, both the proliferation rate of embryonic chick calvarial cells and the growth rate of embryonic chick bone. We now report that the effect of this human skeletal growth factor on chick calvarial cell proliferation in vitro is saturable, prolonged, and inductive. The effect was saturable in that dose/response studies revealed a Kmipp of 12.5 nM/103 cells in a 1-mL volume. The absolute value of