MOLECULAR CHARACTERIZATION OF T-CELL ANTIGEN RECEPTOR EXPRESSION BY SUBSETS OF CD4- CD8- MURINE THYMOCYTES
MOLECULAR CHARACTERIZATION OF T-CELL ANTIGEN RECEPTOR EXPRESSION BY SUBSETS OF CD4- CD8- MURINE THYMOCYTES
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DOI：
10.1073/pnas.85.16.6082
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发表时间：
1988-08-01
影响因子：
11.1
通讯作者：
SHORTMAN, K
中科院分区：
文献类型：
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作者：
PEARSE, M;GALLAGHER, P;SHORTMAN, K
所有T细胞的前体细胞都存在于缺乏CD 4和CD 8表面糖蛋白的胸腺细胞群体中。这些“双阴性”胸腺细胞在其其他表面标志物的表达中是显著异质的，并且包括处于不同发育阶段的细胞。在这项研究中，CD 4-CD 8-成年小鼠胸腺细胞被分成亚群的基础上表达的“热稳定抗原”（HSA）和赖氨酸1（CD 5）。直接分析分选的亚组（没有在培养物中预先扩增）的T细胞抗原受体（TcR）基因重排和mRNA表达以及TcR和CD 3细胞表面蛋白表达。在主要的HSA+ Ly 1 low亚群上检测到非常少的表面CD 3或TcR表达。然而，HSA+ Ly 1high、HSA-Ly 1high和HSA+ Ly 1 low亚群均含有表面表达CD 3和TcR的细胞。与以前的研究相反，我们发现没有一个子集专门表达α。β的或γ。δ的异二聚体，尽管α.β +到. gamma.δ +差别很大。这三个亚群中的两个（HSA-Ly 1低和HSA-Ly 1高）在α中显示非常高的V β 8基因产物的使用。β的异二聚体，但仍然包括。15%非V β 8 α。β的forms.发现所有的CD 4-CD 8-亚群都广泛重排了它们的TcR γ。基因并表达γ。mRNA。表达高比率的成熟[1.3种酶（kg）]与截短的（1.0kb）β。消息和α的存在消息主要限于具有TcR α的子集。β的表面表达
Precursors of all T-lineage cells are found in a population of thymocytes that lack the CD4 and CD8 surface glycoproteins. These "double-negative" thymocytes are markedly heterogeneous in their expression of other surface markers and include cells at various stages of development. In this study, CD4- CD8- adult murine thymocytes were separated into subsets based on the expression of the "heat stable antigen" (HSA) and of Ly 1 (CD5). The sorted subsets were analyzed directly (without prior expansion in culture) for T-cell antigen receptor (TcR) gene rearrangement and mRNA expression and for TcR and CD3 cell-surface protein expression. Very little surface CD3 or TcR expression was detected on the major HSA+ Ly 1low subset. However, the HSA+ Ly 1high, HSA- Ly 1high, and HSA+ Ly 1low subsets all contained cells with surface expression of CD3 and TcR. In contrast to previous studies, we found no subset that exclusively expressed either the .alpha..beta. or .gamma..delta. heterodimer, although the ratio of .alpha..beta.+ to .gamma..delta.+ varied widely. Two of these three subsets (HSA- Ly 1low and HSA- Ly 1high) showed very high usage of V.beta.8 gene products in the .alpha..beta. heterodimer, but nevertheless included .apprxeq. 15% non-V.beta.8 .alpha..beta. forms. All CD4- CD8- subsets were found to have extensively rearranged their TcR.gamma. genes and to express .gamma. mRNA. Expression of a high ratio of mature [1.3 kilobases (kg)] to truncated (1.0 kb) .beta. message and presence of .alpha. message was largely restricted to subsets with TcR .alpha..beta. surface expression.