Intermittent low-level vagosympathetic nerve trunk stimulation inhibits ganglionated plexi activity to prevent atrial fibrillation.
Intermittent low-level vagosympathetic nerve trunk stimulation inhibits ganglionated plexi activity to prevent atrial fibrillation.
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发表时间：
2015-04
影响因子：
0.1
通讯作者：
Yanmei Lu;Juan Sun;Ling Zhang;Qingquan Sun;Xianhui Zhou;Jin-xin Li;Yu Zhang;B. Tang
中科院分区：
文献类型：
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作者：
Yanmei Lu;Juan Sun;Ling Zhang;Qingquan Sun;Xianhui Zhou;Jin-xin Li;Yu Zhang;B. Tang
背景：心脏自主神经系统(CANS)在房颤(房颤)的发生和持续中起着重要作用。低水平迷走神经刺激(LL-VNS)可抑制房颤的发生。目的比较间歇性低水平迷走神经刺激(I-VNS)和持续低水平迷走神经刺激(C-VNS)的效果。方法19只比格犬随机分为3组：A组，快速左心耳起搏6h；B组，快速心房起搏(RAP)6h，C-VNS(20 Hz，间隔0.1ms，方波)，阈值电压50%；C组，RAP 6h，I-VNS(30s开启，30s关闭)。分别于实验开始前、实验开始后1h、3h和6h测定心房单相动作电位(MAP)和心房、肺静脉的有效不应期(ERP)。实验结束后，检测各组大鼠右侧前神经节丛(ARGP)酪氨酸羟化酶(TH)和胆碱乙酰转移酶(ChAT)的表达水平。结果A组MAP、ERP与B、C组比较，差异有统计学意义(P<0.05)。A组MAP和ERP逐渐降低，6h降至最低，B组和C组无明显变化。与A组相比，B组和C组TH和ChAT表达均减少。结论在房颤的发生发展过程中，I-VNS对心血管系统具有保护作用，有可能替代C-VNS。此外，在房颤预防过程中，I-VNS和C-VNS都抑制了神经节丛(GP)的活动。
BACKGROUND The cardiac autonomic nervous system (CANS) plays a role in the occurrence and persistence of atrial fibrillation (AF). Low-level vagosympathetic nerve stimulation (LL-VNS) has been shown to inhibit the occurrence of AF. OBJECTIVE The novel objective of this study was to compare the effects of intermittent low- level vagosympathetic nerve stimulation (I-VNS) and continuous low-level vagosympathetic nerve stimulation (C-VNS). METHODS 19 beagles were randomly divided into 3 groups: Group A, rapid left atrial appendage pacing for 6 hours; Group B, rapid atrial pacing (RAP) for 6 hours and C-VNS (20 Hz, interval 0.1 ms, square wave) with 50% threshold voltage strength; Group C, RAP for 6 hours and I-VNS (continuously recurring cycles of 30-second ON, 30-second OFF). The atrial monophasic action potential (MAP) and the effective refractory periods (ERP) of the atrium and the pulmonary veins were measured at baseline, 1 hour, 3 hours and 6 hours after the experiment began. After the experiment, tyrosine hydroxylase (TH) and choline acetyl transferase (CHAT) expression levels in the anterior right ganglionated plexi (ARGP) from each group were measured. RESULTS Inter-group comparisons of MAP and ERP demonstrated that Group A was significantly different from Groups B and C (P 0.05). The MAP and ERP in Group A gradually decreased, reaching a minimum at 6 hours, but no significant changes were observed in Groups B and C. When compared to Group A, both Groups B and C had reduced TH and CHAT expression. CONCLUSIONS During the occurrence and development of AF, I-VNS could protect the cardiovascular system, possibly replacing C-VNS. Additionally, both I-VNS and C-VNS inhibited ganglionated plexus (GP) activity during the AF prevention.