Alternate junctions and microheterogeneity of Tlr1, a developmentally regulated DNA rearrangement in Tetrahymena thermophila.
Alternate junctions and microheterogeneity of Tlr1, a developmentally regulated DNA rearrangement in Tetrahymena thermophila.
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Tlr1 的交替连接和微观异质性，Tlr1 是嗜热四膜虫中发育调控的 DNA 重排。
DOI：
10.1111/j.1550-7408.1997.tb05733.x
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Karrer,KM
中科院分区：
文献类型：
--
作者：
Patil,NS;Hempen,PM;Udani,RA;Karrer,KM
在嗜热四膜虫大核发育过程中发生了大量发育调控的DNA重排，Tlr1是一种缺失元件，在重排连接附近有大量的反向重复序列，缺失了超过13kbp的内部DNA。先前对核线的分析显示，在B系细胞中，Tlr1重排的左交界处交替。我们在这里展示了C2株四膜虫也使用交替重排连接。我们已经绘制了另外两个重排变体的图谱并进行了测序，发现左和右都可以在大约200个碱基对的范围内变化。我们还证明了在最常见的Tlr1重排产物中存在序列微异质性。
A large number of developmentally regulated DNA rearrangements occur during the development of the macronucleus inTetrahymena thermophila, Tlr1 is a deletion element which has large inverted repeats near the rearrangement junctions and deletes more than 13 kbp of internal DNA. Previous analysis of caryonidal lines revealed alternate left junctions for the Tlr1 rearrangement in B strain cells. We show here that C2 strainTetrahymenaalso use alternate rearrangement junctions. We have mapped and sequenced two additional rearrangement variants and find that both the left and right can vary over a range of approximately 200 bp. We also demonstrate the presence of sequence microheterogeneity in the most commonly found Tlr1 rearrangement product.