Noninvasive photoacoustic identification of sentinel lymph nodes containing methylene blue in vivo in a rat model
Noninvasive photoacoustic identification of sentinel lymph nodes containing methylene blue in vivo in a rat model
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DOI：
10.1117/1.2976427
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发表时间：
2008-09-01
影响因子：
3.5
通讯作者：
Wang, Lihong V.
中科院分区：
文献类型：
--
作者：
Song, Kwang Hyun;Stein, Erich W.;Wang, Lihong V.
前哨淋巴结活检（SLNB）已成为腋窝临床阴性乳腺癌患者腋窝分期的标准方法。尽管使用亚甲蓝和放射性示踪剂的SLNB具有较高的识别率，但它仍然依赖于具有相关发病率的侵入性外科手术。腋窝超声已成为评估腋窝的诊断工具，但它只能评估形态，不能特异性识别前哨淋巴结（SLN）。在这项初步研究中，我们提出了一种非侵入性的光声识别系统，使用亚甲蓝注射在大鼠模型中。我们成功地成像SLN具有高的光学对比度（146 +/- 41，标准差）和良好的超声分辨率（类似于500 μ m）在体内。我们还显示了潜在的可行性，为临床应用成像20-和31-mm深的前哨淋巴结的三维和二维，分别。我们的研究结果表明，这项技术将是一个有用的临床工具，允许临床医生在体内非侵入性地识别前哨淋巴结。(C)2008年，由光学仪器工程师协会（Society of Photo-Optical Instrumentation Engineers）主办。[DOI 10.1117/1.2976427]
Sentinel lymph node biopsy (SLNB) has become the standard method of axillary staging for patients with breast cancer and clinically negative axillae. Even though SLNB using both methylene blue and radioactive tracers has a high identification rate, it still relies on an invasive surgical procedure with associated morbidity. Axillary ultrasound has emerged as a diagnostic tool to evaluate the axilla, but it can only assess morphology and cannot specifically identify sentinel lymph nodes (SLNs). In this pilot study, we propose a noninvasive photoacoustic SLN identification system using methylene blue injection in a rat model. We successfully image a SLN with high optical contrast (146 +/- 41, standard deviation) and good ultrasonic resolution (similar to 500 mu m) in vivo. We also show potential feasibility for clinical applications by imaging 20- and 31-mm-deep SLNs in 3-D and 2-D, respectively. Our results suggest that this technology would be a useful clinical tool, allowing clinicians to identify SLNs noninvasively in vivo. (C) 2008 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.2976427]