Intercellular Accumulation of Type V Collagen Fibrils in Accordance with Cell Aggregation
Intercellular Accumulation of Type V Collagen Fibrils in Accordance with Cell Aggregation
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DOI：
10.1093/jb/mvn109
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发表时间：
2008-11-01
影响因子：
2.7
通讯作者：
Hayashi, Toshihiko
中科院分区：
文献类型：
--
作者：
Kihara, Takanori;Imamura, Yasutada;Hayashi, Toshihiko
我们以前报道过，人成纤维细胞在复盖V型胶原原纤维的培养皿中培养时形成团块。基本上所有最初涂在培养皿上的V型胶原原纤维，都在培养过程中最终形成的细胞团中被恢复。我们解释说，V型胶原原纤维粘附在细胞上比粘附在培养皿上更强，并且随着细胞运动而分离。在这项研究中，V型胶原蛋白与成纤维细胞一起悬浮，以检查V型胶原原纤维的命运，并确定原纤维是否影响细胞直接贴壁的行为。随着成纤维细胞的局部聚集，增加的V型胶原积聚在细胞间隙。扫描电镜检查显示，在没有抗坏血酸的培养细胞附近发现V型胶原原纤维，基本上没有胶原分泌。这些结果表明，V型胶原形成原纤维，原纤维在细胞间隙中积累。积累的V型胶原原纤维作为细胞团块形成的粘合材料。讨论了这种现象与V型胶原原纤维可能参与组织的关系。
We reported previously that human fibroblasts form clumps when cultured on a dish coated with reconstituted type V collagen fibrils. Essentially all the type V collagen fibrils, initially coated on the dish, were recovered in the cell clumps that had eventually formed during the culture. We interpreted that type V collagen fibrils adhere to cells more strongly than to the dish and are detached by cell movements. In this study, type V collagen was suspended with fibroblasts to examine the fate of the type V collagen fibrils and to determine whether the fibrils affect the behaviour of the cells directly adherent to the dish. The added type V collagen accumulated in the intercellular space concomitantly with the local aggregation of fibroblasts. scanning electron microscope examination indicated that type V collagen fibrils were found in the vicinity of cells in cultures without ascorbic acid where essentially no collagen secretion takes place. These results indicate that type V collagen forms fibrils and the fibrils are accumulated in the intercellular spaces. The accumulated type V collagen fibrils work as a cementing material for cell clump formation. This phenomenon is discussed in relation to the possible involvement of type V collagen fibrils in tissue organization.