A Comparison of Stromal Cell-Derived Factor-1 Expression During Distraction Osteogenesis and Bone Fracture in the Mandible
A Comparison of Stromal Cell-Derived Factor-1 Expression During Distraction Osteogenesis and Bone Fracture in the Mandible
复制标题
下颌骨牵张成骨和骨折过程中基质细胞衍生因子 1 表达的比较
DOI：
10.1097/scs.0b013e31828f1ca7
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发表时间：
2013-05-01
影响因子：
0.9
通讯作者：
Lei, De-lin
中科院分区：
文献类型：
--
作者：
Cao, Jian;Wang, Lei;Lei, De-lin
牵张成骨术（Distraction osteogenesis，DO）是一种广泛应用于骨科和颅颌面外科的技术。然而，机械刺激转化为生物信号的确切分子机制仍然知之甚少。在这项研究中，我们检查和比较基质细胞衍生因子-1（SDF-1）的表达在大鼠下颌骨牵张成骨和骨折，分别。将48只雄性SD大鼠随机分为2组，分别在右侧下颌骨截骨后行单侧牵张成骨和坚强内固定。取下颌骨进行放射学、组织学和免疫化学检查。结果发现SDF-1主要表达于成骨细胞和血管中，且在DO区的表达较骨折区强。酶联免疫吸附法定量分析显示，SDF-1在DO中的表达峰值大于骨折愈合中的峰值，表达持续时间长于骨折愈合中的峰值（P < 0.05）。这些结果表明，牵张过程中显着促进SDF-1的高表达，有利于诱导成骨过程中DO。
AbstractDistraction osteogenesis (DO) has been a widely applied technique in orthopedics and craniofacial surgery. However, the exact molecular mechanism by which the mechanical stimulus is translated into biological signals is still poorly understood. In this study, we examined and compared the expression of stromal cell-derived factor-1 (SDF-1) during mandibular distraction osteogenesis and fracture in rats, respectively. Forty-eight male Sprague-Dawley rats were divided into 2 groups and received unilateral distraction osteogenesis and rigid internal fixation, respectively, after the osteotomy on the right mandible. The harvested mandibles were examined radiographically, histologically, and immunohistochemically. We found that the expression of SDF-1 was mainly detected in the osteoblasts and blood vessels, and there were more intensive expression of SDF-1 in DO zones than in bone fracture zones. The quantitative analysis by enzyme-linked immunosorbent assay showed that SDF-1 reached a greater peak and maintained a longer period of up-regulation in DO than in fracture healing (P < 0.05). These results suggest that the distraction procedure markedly promotes the high expression of SDF-1 which facilitates the induction of bone formation during DO.