The effect of castration on calcitonin gene-related peptide in spinal motor neurons.
The effect of castration on calcitonin gene-related peptide in spinal motor neurons.
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去势对脊髓运动神经元降钙素基因相关肽的影响。
DOI：
10.1159/000125243
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发表时间：
1989
影响因子：
4.1
通讯作者：
Micevych,PE
中科院分区：
文献类型：
--
作者：
Popper,P;Micevych,PE
球海绵体脊髓核(SNB)是大鼠脊髓腰段5、6节段约200个雄激素积累运动神经元的性二形态核。在本工作中，我们用免疫组织化学方法研究了长期去势后这些运动神经元中降钙素基因相关肽样免疫反应(CGRP-LI)的变化。在SNB运动神经元的胞体和穿过核团的细小曲张纤维中可见CGRP-LI。在正常动物中，48.4±3.9%(n=8)的黑质神经元呈CGRP-LI阳性，而在去势的动物中，这一比例增加到80.0±1.7%(n=9，p<；0.001)。去势4周后再植入含睾酮硅胶胶囊4周的动物，其含CGRP-LI的SNB运动神经元百分比与完整动物相同。这些结果表明，循环睾酮水平的降低增加了SNB运动神经元中表达CGRP-LI的数量，这意味着雄激素刺激抑制了SNB运动神经元中CGRP-LI的表达。
The spinal nucleus of the bulbocavernosus (SNB) is a sexually dimorphic nucleus of about 200 androgen-accumulating motor neurons in lumbar segments 5 and 6 of the rat spinal cord. In the present work we have used immunohistochemistry to investigate the change in calcitonin gene-related peptide-like immunoreactivity (CGRP-LI) in these motor neurons induced by long-term castration. CGRP-LI was found in the cell bodies of the SNB motor neurons and in thin varicose fibers coursing through the nucleus. In the intact animals 48.4 ± 3.9% (n = 8) of the SNB neurons were CGRP-LI positive while in the castrates this ratio increased to 80.0 ± 1.7% (n = 9, p < 0.001). Animals castrated for 4 weeks and then implanted with testosterone-containing Silastic capsules for an additional 4 weeks had the same percentage of CGRP-LI-containing SNB motor neurons as intact animals. These results demonstrate that a reduction in circulating testosterone levels increases the number of SNB motor neurons that express CGRP-LI implying that androgen stimulation inhibits the expression of CGRP-LI in SNB motor neurons.