Biocorrosion and uptake of titanium by human osteoclasts
Biocorrosion and uptake of titanium by human osteoclasts
复制标题
DOI：
10.1002/jbm.a.32914
复制
发表时间：
2010-12-15
影响因子：
4.9
通讯作者：
Filgueira, Luis
中科院分区：
文献类型：
--
作者：
Cadosch, Dieter;Al-Mushaiqri, Mohamed S.;Filgueira, Luis
所有与生物系统接触的金属都会通过电化学氧化还原反应发生腐蚀。本研究调查了人类破骨细胞（OC）是否能够在钛和铝上生长，并直接腐蚀金属，导致相应的金属离子释放，这些金属离子被认为会引起炎症反应并激活骨细胞分化。扫描电子显微镜分析表明，长期可行的OC文化的表面上的钛和铝箔。原子发射光谱法研究表明，铝上培养的OC上清液中铝的水平显着增加，然而，在钛上的细胞培养物的上清液中的所有测量值均低于检测限。尽管如此，用纽波特绿色DCF二乙酸酯染色的共聚焦显微镜分析描绘了在钛箔上培养的OC的整个细胞质和核仁中的强烈荧光。在玻璃上培养的单核细胞和对照细胞中未观察到相当的荧光强度。目前的研究表明，人破骨细胞前体细胞能够在钛和铝上生长并向成熟OC分化。此外，它确定了成熟细胞能够直接腐蚀金属表面并吸收相应的金属离子，随后可能释放金属离子，从而诱导假体周围骨中溶骨性病变的形成，导致植入物松动。(C)2010 Wiley Periodicals，Inc. J Biomed Mater Res Part A：95A：1004- 1010，2010.
All metals in contact with a biological system undergo corrosion through an electrochemical redox reaction. This study investigated whether human osteoclasts (OC) are able to grow on titanium and aluminum, and directly corrode the metals leading to the release of corresponding metal ions, which are believed to cause inflammatory reactions and activate osteoclastic differentiation. Scanning electron microscopy analysis demonstrated long-term viable OC cultures on the surface of titanium and aluminum foils. Atomic emission spectrometry investigations showed significantly increased levels of aluminum in the supernatant of OC cultured on aluminum; however, all measurements in the supernatants of cell cultures on titanium were below detection limits. Despite this, confocal microscopy analysis with Newport Green DCF diacetate ester staining depicted intense fluorescence throughout the cytoplasm and nucleolus of OC cultured on titanium foils. Comparable fluorescence intensities were not observed in monocytes and control cells cultured on glass. The present study demonstrated that human osteoclast precursors are able to grow and differentiate toward mature OC on titanium and aluminum. Furthermore, it established that the mature cells are able to directly corrode the metal surface and take up corresponding metal ions, which subsequently may be released and thereby induce the formation of osteolytic lesions in the periprosthetic bone, contributing to the loosening of the implant. (C) 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 95A: 1004-1010,2010.