Change in transferrin sialylation is a potential prognostic marker for severity of acute pancreatitis
Change in transferrin sialylation is a potential prognostic marker for severity of acute pancreatitis
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DOI：
10.1016/j.clinbiochem.2008.01.026
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发表时间：
2008-05-01
影响因子：
2.8
通讯作者：
Lauc, Gordan
中科院分区：
文献类型：
--
作者：
Gornik, Olga;Gornik, Ivan;Lauc, Gordan
目的：重症急性胰腺炎的早期预测是临床实践中的问题之一。由于许多疾病都与糖基化的改变有关，因此在这项工作中，我们研究了急性胰腺炎中转铁蛋白和血清蛋白的唾液酸化。设计和方法：对 30 名患者住院前 8 天的唾液酸化进行了分析，并与 28 名健康对照进行了比较。使用酶联凝集素测定法测量转铁蛋白唾液酸化，使用间苯二酚法测量蛋白质上的唾液酸。结果：两个分析参数在研究期间都发生了变化。住院第1天和第2天转铁蛋白唾液酸化的变化被证明是急性胰腺炎的早期预后标志物，其敏感性（88.9%）和特异性（90.5%）优于其他测试标志物。结论：转铁蛋白和血清总蛋白的唾液酸化反应反映了急性胰腺炎期间炎症反应的强度，可作为疾病严重程度的预后参数。 (c) 2008 年加拿大临床化学家协会。由爱思唯尔公司出版。保留所有权利。
Objectives: Early prediction of severe acute pancreatitis is one of the problems in clinical practice. Since many diseases are associated with alteration in glycosylation, in this work we studied sialylation of transferrin and serum proteins in acute pancreatitis.Design and methods: Sialylation was analyzed during first eight days of hospitalization of 30 patients and compared to 28 healthy controls. Transferrin sialylation was measured using enzyme linked lectin assay, while sialic acid on proteins was measured using resorcinol method.Results: Both analyzed parameters changed during studied period. The change in transferrin sialylation between Day1 and Day2 of hospitalization was shown to be an early prognostic marker of acute pancreatitis, with better sensitivity (88.9%) and specificity (90.5%) than other markers tested.Conclusions: Sialylation of transferrin and total serum proteins reflects the intensity of inflammatory response during acute pancreatitis and could be used as prognostic parameter for disease severity. (c) 2008 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.