Effect of pullulan hydrolysates on the quality of Nile tilapia (Oreochromis niloticus) fillets during ice storage
Effect of pullulan hydrolysates on the quality of Nile tilapia (Oreochromis niloticus) fillets during ice storage
复制标题
普鲁兰多糖水解物对冰藏期间尼罗罗非鱼（Oreochromis niloticus）鱼片品质的影响
DOI：
10.1111/jfpp.14043
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发表时间：
2019-08-01
影响因子：
2.5
通讯作者：
Cheng, Chunsheng
中科院分区：
文献类型：
--
作者：
Chen, Hui;Wu, Yongmin;Cheng, Chunsheng
利用巨大芽孢杆菌产普鲁兰酶水解普鲁兰多糖。当水解物浓度达到30 mg/ml时，其ABTS（+）、OH和DPPH自由基清除率分别为37.73%、21.67%和17.03%，显著高于普鲁兰多糖（p0.05）。以普鲁兰多糖水解物为基料制备可食性涂膜剂，对尼罗罗非鱼片进行冰藏保鲜。结果表明：2%和3%的水解物对降低2-硫代巴比妥酸（TBA）、总挥发性盐基氮（TN）、电导率、pH值、失水率和变色率等理化指标，抑制细菌生长效果最好;与对照样品相比，（p < 0.05），两组间无显著性差异（p > 0.05）。实际应用基于多糖的可食性涂膜是一种理想的鱼片保鲜方法。结果表明，普鲁兰多糖酶解物具有较好的延长鱼片货架期的作用。本研究为尼罗罗非鱼片的质量控制提供了有效的方法。
Pullulan was hydrolyzed by pullulanase producted by Bacillus megaterium. The ABTS(+), OH, and DPPH scavenging activities of hydrolysates were 37.73, 21.67, and 17.03%, respectively, when its concentration reached 30 mg/ml, significantly higher than pullulan (p 0.05). Edible coatings based on pullulan hydrolysates were used to preserve Nile tilapia (Oreochromis niloticus) fillets under ice storage. The results showed that 2 and 3% hydrolysates were most effective in decreasing physicochemical changes including 2-thiobarbituric acid (TBA), total volatile basic nitrogen, electrical conductivity, pH, water loss, and discoloration, inhibiting bacterial growth; and increasing the sensory quality of fish fillets compared with control samples (p < 0.05), and no significant differences were observed between them (p > 0.05). Practical applications Edible coating based on polysaccharide is an ideal method of fish fillets preservation. Results indicated that pullulan hydrolysates has good ability on extending shelf life of fish fillets. This study could provide a efficient method on the quality control of Nile tilapia fillets.