Fatty acid moieties have little effect on cholesterol‐lowering potency of plant sterol esters
Fatty acid moieties have little effect on cholesterol‐lowering potency of plant sterol esters
复制标题
DOI：
10.1002/ejlt.201400444
复制
发表时间：
2015-05
影响因子：
2.7
通讯作者：
Yuwei Liu;Lei Guan;Youyou Zhao;Lin Lei;Xiaobo Wang;K. Ma;Y. Li;Lijun Wang;Sun Wa Man;Junkuan Wang;Yu Huang;Zhen-Yu Chen
中科院分区：
文献类型：
--
作者：
Yuwei Liu;Lei Guan;Youyou Zhao;Lin Lei;Xiaobo Wang;K. Ma;Y. Li;Lijun Wang;Sun Wa Man;Junkuan Wang;Yu Huang;Zhen-Yu Chen
植物甾醇酯是流行的降胆固醇膳食补充剂。为了了解脂肪酸部分在植物甾醇酯降胆固醇活性中的作用，本研究旨在：(1)比较葵花籽油(SSF)和菜籽油(SCO)甾醇酯的降胆固醇活性；(2)系统研究葵花籽油(SSF)和菜籽油(SCO)对高脂饮食金黄地鼠胆固醇转运体、酶、受体和胆固醇代谢相关蛋白基因表达的影响。GC分析表明，SSF和SCO具有相似的甾醇组成，但具有不同的脂肪酸组成。结果表明，SSF和SCO均能以剂量依赖的方式降低血浆胆固醇。SSF和SCO均能抑制胆固醇的吸收，促进胆汁酸的排泄。日粮中添加SSF和SCO均可上调肝脏胆固醇-7-羟基酶(α-7A1)，下调肠道固醇转运蛋白NPC1L1、酰基辅酶A：胆固醇酰基转移酶2(ACAT2)和肝脏3-羟基-3-甲基戊二酰辅酶A还原酶(HMG-CoA还原酶)的表达。由此可见，脂肪酸基对SSF和SCO的降胆固醇活性影响不大。实际应用：植物甾醇能有效降低血浆胆固醇。商业上，葵花籽油的植物甾醇酯和菜籽油的植物甾醇酯主要用于此目的。目前的研究发现，SSF和SCO在降低血浆胆固醇方面同样有效，并呈剂量依赖关系，尽管它们的脂肪酸组成存在显着差异。脂肪酸部分对植物甾醇酯的降胆固醇效力几乎没有影响。
Plant sterol esters are the popular cholesterol-lowering dietary supplements. In order to understand the role of fatty acid moieties in the cholesterol-lowering activity of plant sterol esters, the present study was to: (i) compare the cholesterol-lowering activity of sterol esters of sunflower oil (SSF) with that of sterol esters of canola oil (SCO); and (ii) to systematically investigate the effect of SSF and SCO on the gene expression of sterol transporters, enzymes, receptors and proteins involved in cholesterol metabolism in hamsters fed a high cholesterol diet. GC analysis showed that SSF and SCO had similar sterol profile but they had different fatty acid moieties. Results showed both SSF and SCO could equally reduce plasma cholesterol in a dose-dependent manner. Both SSF and SCO could inhibit the cholesterol absorption and enhance the excretion of bile acids. In the similar ways, dietary SSF and SCO could up-regulate hepatic cholesterol-7α-hydroxylase (CYP7A1) and down-regulate intestinal sterol transporter Niemann-Pick C1-like 1 (NPC1L1), acyl coenzyme A: cholesterol acyltransferase 2 (ACAT2) and hepatic 3-hydroxy-3-methylglutaryl-CoA reductase (HMG-CoA reductase). It was concluded that the fatty acid moieties had little effect on the cholesterol-lowering activity of SSF and SCO. Practical applications: Plant sterols are effective in reducing plasma cholesterol. Commercially, plant sterol esters of sunflower oil and plant sterol esters of canola oil are mostly used for this purpose. The present study found that SSF and SCO were equally effective in reducing plasma cholesterol in a dose-dependent manner regardless the significant difference in their fatty acid compositions. Fatty acid moieties have little effect on cholesterol-lowering potency of plant sterol esters.