Morphology, Physiological Characteristics, and Complete Sequence of Marine Bacteriophage φRIO-1 Infecting Pseudoalteromonas marina
Morphology, Physiological Characteristics, and Complete Sequence of Marine Bacteriophage φRIO-1 Infecting Pseudoalteromonas marina
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DOI：
10.1128/jvi.01521-13
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发表时间：
2013-08-01
影响因子：
5.4
通讯作者：
Cho, Byung C.
中科院分区：
文献类型：
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作者：
Hardies, Stephen C.;Hwang, Yeon J.;Cho, Byung C.
假交替单胞菌属细菌在世界海洋中普遍存在。海洋细菌被认为与 T7 相关的足病毒的一个主要古老分支有关。然而，尽管已经报道了属于皮质病毒科和Siphoviridae的假交替单胞菌噬菌体以及属于肌病毒科的原噬菌体，但之前尚未知道假交替单胞菌足病毒。在这里，分离出一种新的裂解性假交替单胞菌滨海噬菌体，phi RIO-1，属于足病毒科，并对其形态、基因组序列和生物学特性进行了表征。它的主要编码蛋白与 T7 非常相似。先前测序的最相似病毒是假单胞菌噬菌体 PA11 和盐弧菌噬菌体 CW02。尽管phi RIO-1的形态学和基因组织的许多元素与与T7广泛相关的足病毒相似，但phi RIO-1明显缺乏RNA聚合酶基因。由于 T7 超群的定义包括 DNA 聚合酶基因的相似性，因此进行了详细的系统发育分析，发现了自写病毒亚科中的两个主要 DNA 聚合酶分支和足病毒中代表的 polA 家族的几个结构变体。 phi RIO-1 携带的操纵子与其他一些足病毒中的操纵子类似，预计可指定与 γ-谷氨酰胺键和/或异常肽键相关的活性。除了较长的潜伏期外，phi RIO-1 的大多数生长特性都是 T7 样噬菌体的典型特性。
Bacteria of the genus Pseudoalteromonas are ubiquitous in the world's oceans. Marine bacteria have been posited to be associated with a major ancient branch of podoviruses related to T7. Yet, although Pseudoalteromonas phages belonging to the Corticoviridae and the Siphoviridae and prophages belonging to the Myoviridae have been reported, no Pseudoalteromonas podovirus was previously known. Here, a new lytic Pseudoalteromonas marina phage, phi RIO-1, belonging to the Podoviridae was isolated and characterized with respect to morphology, genomic sequence, and biological properties. Its major encoded proteins were distantly similar to those of T7. The most similar previously sequenced viruses were Pseudomonas phage PA11 and Salinivibrio phage CW02. Whereas many elements of the morphology and gene organization of phi RIO-1 are similar to those of podoviruses broadly related to T7, phi RIO-1 conspicuously lacked an RNA polymerase gene. Since definitions of a T7 supergroup have included similarity in the DNA polymerase gene, a detailed phylogenetic analysis was conducted, and two major DNA polymerase clades in Autographivirinae and several structural variants of the polA family represented in podoviruses were found. phi RIO-1 carries an operon similar to that in a few other podoviruses predicted to specify activities related to gamma-glutamyl amide linkages and/or unusual peptide bonds. Most growth properties of phi RIO-1 were typical of T7-like phages, except for a long latent period.