Establishment of a new methicillin resistant staphylococcus aureus animal model of osteomyelitis
Establishment of a new methicillin resistant staphylococcus aureus animal model of osteomyelitis
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DOI：
10.1007/s00264-013-2149-1
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发表时间：
2014-04
影响因子：
2.7
通讯作者：
L. Helbig;H. Simank;H. Lorenz;C. Putz;C. Wölfl;A. Suda;A. Moghaddam;G. Schmidmaier;T. Guehring
中科院分区：
文献类型：
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作者：
L. Helbig;H. Simank;H. Lorenz;C. Putz;C. Wölfl;A. Suda;A. Moghaddam;G. Schmidmaier;T. Guehring
目的耐甲氧西林金黄色葡萄球菌 (MRSA) 感染的增加目前是一个主要的卫生保健问题。万古霉素仍然常常是治疗此类感染的一线抗微生物药物；然而，最近万古霉素治疗 MRSA 感染的疗效下降引起了人们的关注，并加速了新抗生素的寻找。本研究的目的是建立 MRSA 种植体周围骨髓炎动物模型，以便将来在典型 MRSA 感染条件下测试新型抗微生物药物。方法 18 只随机 NZW 兔接受了插入股骨骨植入物的标准化手术程序。然后将动物分为第1组（MRSA接种，无抗生素；M/N）、第2组（MRSA接种，万古霉素；M/V）和第3组（无MRSA接种，无抗生素；N/N）。主要研究结果参数是术后 1 天、7 天和 42 天的动物白细胞计数、动物体重和动物体温。此外，还建立了组织形态计量评分并调整为修改后的组织学 Smeltzer 评分。结果宏观和组织形态计量结果显示，与 M/V 和 N/N 相比，第 1 组存在种植体周围骨髓炎，M/N 中的急性和慢性感染参数均增加，表明万古霉素治疗可预防 MRSA 种植体周围骨髓炎的典型形态学变化。类似地，第 1 组动物体重减少，白细胞计数和体温增加（每个 p< 0.005）。万古霉素治疗再次导致白细胞计数和体温显着降低，动物体重增加。结论我们建立了种植体周围 MRSA 骨髓炎模型，该模型成功地将感染的临床和实验室结果参数与组织形态测量结果相结合；该模型似乎对于未来新型抗微生物 MRSA 药物的实验使用和治疗监测很有价值。
PurposeThe increase in methicillin-resistantStaphylococcus aureus(MRSA) infections is currently a major health care problem. Vancomycin is still often the first-line anti-microbiological agent for treating such infections; however, a recent decline in efficacy of vancomycin in MRSA infections has raised concerns and accelerated the search for new antibiotics. The aim of this study was to establish a MRSA peri-implant osteomyelitis animal model for future testing of new anti-microbiological agents under typical MRSA infection conditions.MethodsEighteen randomised NZW-rabbits underwent a standardised surgical procedure with the insertion of a femoral bone implant. Animals were then divided into group 1 (MRSA inoculation, no antibiotics; M/N), group 2 (MRSA inoculation, Vancomyin; M/V), and group 3 (no MRSA inoculation, no antibiotics; N/N). The primary study outcome parameters were animal leucocyte count, animal weight, and animal body temperature at one, seven, and 42 days after surgery. Additionally, a histo-morphometrical score was established and adjusted to a modified histological Smeltzer score.ResultsMacroscopic and histo-morphometrical findings showed a peri-implant osteomyelitis in group 1 with both increased acute and chronic infection parameters in M/N, as compared to M/V and N/N, indicating that vancomycin treatment prevented typical morphological changes of MRSA peri-implant osteomyelitis. Similarly, there was a reduction in animal weight and increase in leucocyte count and body temperature in group 1 (eachp< 0.005). Vancomycin treatment again resulted in significantly reduced leucocyte count and body temperature, and increased animal body weight.ConclusionsHere we have established a peri-implant MRSA osteomyelitis model that successfully combined clinical and laboratory outcome parameters of infection with histo-morphometrical results; this model appears to be valuable for future experimental use and therapeutic monitoring of new anti-microbiological MRSA drugs.