PKG in Honey Bees: Spatial Expression, Amfor Gene Expression, Sucrose Responsiveness, and Division of Labor
PKG in Honey Bees: Spatial Expression, Amfor Gene Expression, Sucrose Responsiveness, and Division of Labor
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DOI：
10.1002/cne.23500
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发表时间：
2014-06-01
影响因子：
2.5
通讯作者：
Scheiner, Ricarda
中科院分区：
文献类型：
--
作者：
Thamm, Markus;Scheiner, Ricarda
分工是群居昆虫的一个标志。在蜜蜂中，劳动分工涉及女工从一项任务到下一项任务的过渡。最不同的任务是护理（为育雏提供食物）和觅食（收集花粉和花蜜）。调节这种行为可塑性的大脑机制在很大程度上仍然是难以捉摸的。最近，有人提出，劳动分工是基于营养相关的信号通路。一个高度保守的基因与跨物种的食物相关的行为是觅食基因，它编码环磷酸鸟苷（cGMP）依赖性蛋白激酶（PKG）。我们对该基因的分析揭示了蜜蜂中存在选择性剪接。一种亚型在脑中表达。这种亚型的表达是最明显的蘑菇体，食管下神经节，和咽侧体。蜜蜂的劳动分工和蔗糖反应与不同脑区的觅食基因表达显著相关。激活PKG选择性地增加蔗糖的反应，在护士蜜蜂觅食的水平，而同样的治疗不影响对光的反应。这些发现证明了不同脑区PKG信号与劳动分工之间的直接联系。此外，他们还证明，可以通过激活PKG来补偿保育蜂和觅食蜂之间的感觉反应差异。我们的研究结果PKG在调节特定的感觉反应和社会组织的功能提供了宝贵的指示cGMP/PKG途径在许多其他昆虫和脊椎动物的功能。J. Comp.神经元522：1786-1799，2014. (c)2013 Wiley Periodicals，Inc.
Division of labor is a hallmark of social insects. In honey bees, division of labor involves transition of female workers from one task to the next. The most distinct tasks are nursing (providing food for the brood) and foraging (collecting pollen and nectar). The brain mechanisms regulating this form of behavioral plasticity have largely remained elusive. Recently, it was suggested that division of labor is based on nutrition-associated signaling pathways. One highly conserved gene associated with food-related behavior across species is the foraging gene, which encodes a cyclic guanosine monophosphate (cGMP)-dependent protein kinase (PKG). Our analysis of this gene reveals the presence of alternative splicing in the honey bee. One isoform is expressed in the brain. Expression of this isoform is most pronounced in the mushroom bodies, the subesophageal ganglion, and the corpora allata. Division of labor and sucrose responsiveness in honey bees correlate significantly with foraging gene expression in distinct brain regions. Activating PKG selectively increases sucrose responsiveness in nurse bees to the level of foragers, whereas the same treatment does not affect responsiveness to light. These findings demonstrate a direct link between PKG signaling in distinct brain areas and division of labor. Furthermore, they demonstrate that the difference in sensory responsiveness between nurse bees and foragers can be compensated for by activating PKG. Our findings on the function of PKG in regulating specific sensory responsiveness and social organization offer valuable indications for the function of the cGMP/PKG pathway in many other insects and vertebrates. J. Comp. Neurol. 522:1786-1799, 2014. (c) 2013 Wiley Periodicals, Inc.