Effect of dietary vitamin E on expiration of pentane and ethane by the rat
Effect of dietary vitamin E on expiration of pentane and ethane by the rat
复制标题
膳食维生素E对大鼠戊烷和乙烷呼气的影响
作者：
C. Dillard;E. Dumelin;A. Tappel
本文介绍了一种测定大鼠呼气中烃类气体的分析方法。该方法用于跟踪体内形成的氢过氧化物的断裂产物在大鼠呼吸中的呼气。主要产物是亚油酸家族的戊烷和亚麻酸家族的乙烷。大鼠以0、11或40 i.u.维生素E乙酸酯/kg饮食，从21日龄开始7周。通过呼气样本的气相色谱分析获得的数据通过Mann-Whitney非参数U检验进行分析。该统计分析表明，在1-7周期间，未补充维生素E的大鼠组所产生的戊烷显著高于两个补充组大鼠中的任何一个。未补充组的乙烷显著高于补充40 i.u.维生素E/kg饲料的添加量在第5周时显著高于两个添加组，第6周时显著高于两个添加组。到7周时，戊烷的生产是10倍以上的nonsupplemented组比在任何补充组，乙烷是约两倍。补充11和40 i.u.乙烷或戊烷的维生素E/kg饮食。这种新的技术，它测量体内脂质过氧化的断裂产物，有望适用于许多实验领域，脂质过氧化是预期或已知发生。
An analytical method for the measurement of hydrocarbon gases in the breath of rats is described. The method was used to follow the expiration in rat breath of in vivo formed scission products of hydroperoxides. The major products are pentane from the linoleic acid family and ethane from the linolenic acid family. Rats were fed 0, 11 or 40 i.u. vitamin E acetate/kg diet for 7 wk starting at age 21 days. Data obtained by gas chromatographic analysis of breath samples were analyzed by the Mann-Whitney nonparametricU-test. This statistical analysis showed that pentane evolved by the group of rats not supplemented with vitamin E was significantly higher during the period 1–7 wk than that evolved by either of the two supplemented groups of rats. Ethane from the nonsupplemented group was significantly higher than that from the group supplemented with 40 i.u. vitamin E/kg of diet by 5 wk, and significantly higher than both supplemented groups by 6 wk. By 7 wk, pentane production was tenfold greater in the nonsupplemented group than in either supplemented group, and ethane was about twofold greater. There was no significant difference between the groups supplemented with 11 and 40 i.u. vitamin E/kg diet for either ethane or pentane. This new technique, which measures scission products from in vivo lipid peroxidation, promises to be useful for application to many experimental areas where lipid peroxidation is expected or known to occur.