Tyrosine hydroxylase in rat brain--cofactor requirements, regional and subcellular distribution.
Tyrosine hydroxylase in rat brain--cofactor requirements, regional and subcellular distribution.
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大鼠脑中酪氨酸羟化酶——辅因子需求、区域和亚细胞分布。
DOI：
10.1016/0006-2952(72)90006-8
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发表时间：
1972
影响因子：
5.8
通讯作者：
J. Coyle
中科院分区：
文献类型：
--
作者：
J. Coyle
本文介绍了一种灵敏的大鼠脑内酪氨酸羟基酶的放射化学分析方法。该酶受过氧化氢酶、二氢蝶啶还原酶和亚铁离子的刺激，在含有Triton X-100的低渗缓冲液中均质时酶活最高。在饱和浓度的l-酪氨酸下，可以检测到毫克量的大鼠脑组织。亚细胞分布研究表明，该酶主要集中在神经末梢部位。低渗休克可将70%的活性释放为可溶组分。
A sensitive, radiochemical assay for tyrosine hydroxylase in rat brain is described. The enzyme is stimulated by catalase, dihydropteridine reductase and ferrous ion; maximal activity was found by homogenization in hypotonie buffer containing Triton X-100. Milligram quantities of rat brain tissue could be assayed at saturating concentrations ofl-tyrosine. The enzyme is concentrated in the nerve terminal fractions as demonstrated by subcellular distribution studies. Seventy per cent of the activity can be released into the soluble fractions by hypotonie shock.