The effect of dietary asparagine supplementation on energy metabolism in liver of weaning pigs when challenged with lipopolysaccharide.
The effect of dietary asparagine supplementation on energy metabolism in liver of weaning pigs when challenged with lipopolysaccharide.
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DOI：
10.5713/ajas.17.0426
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发表时间：
2018-04
期刊：
影响因子：
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通讯作者：
Hou Y
中科院分区：
文献类型：
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作者：
Kang P;Liu Y;Zhu H;Zhang J;Shi H;Li S;Pi D;Leng W;Wang X;Wu H;Hou Y
本试验旨在探讨天冬酰胺(ASN)能否改善脂多糖刺激下断奶仔猪的肝脏能量状况。选用48头断奶仔猪(杜×大×长，体重8.12±0.56 kg)，随机分为4组：(1)CTRL组，饲喂对照日粮，注射无菌生理盐水；(2)脂多糖激发对照组(LPSCC)，饲喂相同对照日粮，注射大肠杆菌脂多糖；(3)脂多糖(脂多糖)+0.5%ASN组，饲喂0.5%ASN组，注射脂多糖；(Iv)脂多糖+1.0%ASN组，饲喂1.0%ASN组，注射脂多糖。所有仔猪均饲喂试验日粮19d，第20天，在试验基础上，分别以100μg/kg体重或等体积的0.9%氯化钠溶液给仔猪腹腔注射大肠杆菌内毒素。分别于注射脂多糖或生理盐水后4h和2 4h处死动物，取肝组织标本。在内毒素攻击后24 h，添加0.5%的天冬氨酸可增加肝脏中的ATP浓度(二次方，p<；0.05)，并有增加腺苷能荷，降低AMP/ATP比值(二次，p<；0.1)的趋势。此外，ASN还增加了肝脏丙酮酸激酶、丙酮酸脱氢酶、柠檬酸合成酶和异柠檬酸脱氢酶β的基因表达(线性，p<；0.05；二次，p<；0.05)，并有增加己糖激酶2mRNA表达的趋势(线性，p<；0.1)。此外，ASN还增加了肝脏磷酸化AMP活化蛋白激酶(PAMPK)/总AMPK(TAMPK)的比率(线性，p<；0.05；二次，p<；0.05)。然而，在内毒素攻击后4h，补充ASN对这些参数没有影响。本研究提示ASN可改善内毒素攻击后期损伤肝脏的能量代谢。
This experiment was conducted to investigate whether asparagine (Asn) could improve liver energy status in weaning pigs when challenged with lipopolysaccharide. Forty-eight weaned pigs (Duroc×Large White×Landrace, 8.12±0.56 kg) were assigned to four treatments: i) CTRL, piglets received a control diet and injected with sterile 0.9% NaCl solution; ii) lipopolysaccharide challenged control (LPSCC), piglets received the same control diet and injected with Escherichia coli LPS; iii) lipopolysaccharide (LPS)+0.5% Asn, piglets received a 0.5% Asn diet and injected with LPS; and iv) LPS+1.0% Asn, piglets received a 1.0% Asn diet and injected with LPS. All piglets were fed the experimental diets for 19 d. On d 20, the pigs were injected intraperitoneally with Escherichia coli LPS at 100 μg/kg body weights or the same volume of 0.9% NaCl solution based on the assigned treatments. Then the pigs were slaughtered at 4 h and 24 h after LPS or saline injection, and the liver samples were collected. At 24 h after LPS challenge, dietary supplementation with 0.5% Asn increased ATP concentration (quadratic, p<0.05), and had a tendency to increase adenylate energy charges and reduce AMP/ATP ratio (quadratic, p<0.1) in liver. In addition, Asn increased the liver mRNA expression of pyruvate kinase, pyruvate dehydrogenase, citrate synthase, and isocitrate dehydrogenase β (linear, p<0.05; quadratic, p<0.05), and had a tendency to increase the mRNA expression of hexokinase 2 (linear, p<0.1). Moreover, Asn increased liver phosphorylated AMP-activated protein kinase (pAMPK)/total AMP-activated protein kinase (tAMPK) ratio (linear, p<0.05; quadratic, p<0.05). However, at 4 h after LPS challenge, Asn supplementation had no effect on these parameters. The present study indicated that Asn could improve the energy metabolism in injured liver at the late stage of LPS challenge.