sKlotho is associated with the severity of brick tea-type skeletal fluorosis in China
sKlotho is associated with the severity of brick tea-type skeletal fluorosis in China
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sKlotho与中国砖茶型氟骨症的严重程度相关
DOI：
10.1016/j.scitotenv.2020.140749
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发表时间：
2020-11-20
影响因子：
9.8
通讯作者：
Sun, Dianjun
中科院分区：
文献类型：
--
作者：
Liu, Yang;Yang, Yanmei;Sun, Dianjun
血清可溶性Klotho （sKlotho）含量的变化与多种骨关节病有关。然而，其与氟骨症（SE）严重程度的关系尚不清楚。本文研究了茶氟暴露与血清sKlotho水平和SF严重程度的关系，并在氟中毒大鼠模型中进一步验证了这一关系。对中国青海和新疆两省50岁以上的砖茶饮用地区的居民进行了横断面病例对照研究。采用离子选择电极法测定砖茶水和尿液中氟化物浓度，ELISA法测定血清sKlotho水平。建立线性回归和有序logistic回归模型，探讨氟暴露、血清sKlotho水平与SF严重程度的关系。方法分离Wistar大鼠肾脏和小肠，采用免疫组化（immunohistochemistry， INC）法检测Klotho，取股动脉血测定血清中sKlotho的水平。茶氟摄入量（TFI）每增加1毫克/天，血清sKlotho水平增加12.070 pg/mL (95% CI: 0.452-23.689)， SF严重程度在调整年龄、性别和种族后增加1.163倍（95% CI: 1.007-1.342）。Scrum sKlotho水平也与SF的严重程度呈正相关（P < 0.05）。中介分析显示，TFI每增加1 mg/d，血清sKlotho水平介导了SF严重程度增加的17.76%。此外，在大鼠模型中，氟暴露组血清sKlotho水平也显著升高。本研究提示血清sKlotho可能是砖茶型氟中毒流行地区SF的潜在介质。(C) 2020年Elsevier B.V.出版
The change of serum soluble Klotho (sKlotho) content is related to a variety of osteoarthropathy. However, its association with the severity of skeletal fluorosis (SE) is not clear. Here, the association of tea fluoride exposure with serum sKlotho levels and the severity of SF were investigated and further verified in a rat model of fluorosis. A cross sectional case control study was conducted in residents over 50 years old from brick-tea drinking areas in Qinghai and Xinjiang Provinces, China. Concentrations of fluoride in brick tea water and urine were determined by ion selective electrode method, and the levels of serum sKlotho were determined by ELISA method. Linear regression and ordered logistic regression models were constructed to examine the relationship among fluoride exposure, serum sKlotho levels and the severity of SF. The kidney and small intestine of Wistar rats were isolated for detection of Klotho by immunohistochemistry (INC), and femoral artery blood was sampled to measure the serum levels of sKlotho. An increase of 1 mg/day in tea fluoride intake (TFI) was associated with a 12.070 pg/mL (95% CI: 0.452-23.689) increase in serum sKlotho levels and a 1.163-fold (95% CI: 1.007-1.342) increase in the severity of SF after adjusting for age, gender, and ethnicity. Scrum sKlotho levels were also positively associated with the severity of SF (P < 0.05). The mediation analysis showed that serum sKlotho levels mediated 17.76% of the increase in the severity of SF caused by an increase of 1 mg/day of TFI. Moreover, a significant increase of serum sKlotho levels in fluoride-exposed groups was also seen in the rat model. The present study suggests that serum sKlotho may be a potential mediator of SF in brick tea-type fluorosis endemic areas. (C) 2020 Published by Elsevier B.V.