Increased Cell Proliferation and Gene Expression of Genes Related to Bone Remodeling, Cell Adhesion and Collagen Metabolism in the Periodontal Ligament of Unopposed Molars in Growing Rats.
Increased Cell Proliferation and Gene Expression of Genes Related to Bone Remodeling, Cell Adhesion and Collagen Metabolism in the Periodontal Ligament of Unopposed Molars in Growing Rats.
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DOI：
10.3389/fphys.2017.00075
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发表时间：
2017
影响因子：
4
通讯作者：
Kiliaridis S
中科院分区：
文献类型：
--
作者：
Dorotheou D;Farsadaki V;Bochaton-Piallat ML;Giannopoulou C;Halazonetis TD;Kiliaridis S
牙齿萌出是牙齿从牙槽骨内涌入口腔的过程，人们对此知之甚少。牙齿萌出的后遗症会持续一生，特别是在牙齿没有被拮抗者反对的情况下。本研究的目的是为了更好地了解萌出后牙萌出的分子过程。为此，我们摘除了14只年轻大鼠口腔一侧上颌磨牙的牙冠，并在3天和15天后检测了同侧和对侧下颌磨牙牙周韧带(PDL)中的基因表达模式。9只未经处理的大鼠作为对照。6个基因Adamts18、Ostn、P4ha3、Panx3、Pth1r和Tnmd在非对位磨牙中表达上调。这些基因在成骨细胞的分化和增殖、细胞黏附和胶原代谢中发挥作用。随着拮抗牙的丧失，PDL细胞的增殖也增加。有趣的是，PTH1R的突变与人类牙萌出后阶段的缺陷有关。我们得出结论，非对立牙齿的萌出后萌出与有利于牙槽骨形成和牙周韧带重塑的基因表达模式有关。
Tooth eruption, the process by which teeth emerge from within the alveolar bone into the oral cavity, is poorly understood. The post-emergent phase of tooth eruption continues throughout life, in particular, if teeth are not opposed by antagonists. The aim of the present study was to better understand the molecular processes underlying post-emergent tooth eruption. Toward this goal, we removed the crowns of the maxillary molars on one side of the mouth of 14 young rats and examined gene expression patterns in the periodontal ligaments (PDLs) of the ipsilateral and contralateral mandibular molars, 3 and 15 days later. Nine untreated rats served as controls. Expression of six genes, Adamts18, Ostn, P4ha3, Panx3, Pth1r, and Tnmd, was upregulated in unopposed molars relative to molars with antagonists. These genes function in osteoblast differentiation and proliferation, cell adhesion and collagen metabolism. Proliferation of PDL cells also increased following loss of the antagonist teeth. Interestingly, mutations in PTH1R have been linked to defects in the post-emergent phase of tooth eruption in humans. We conclude that post-emergent eruption of unopposed teeth is associated with gene expression patterns conducive to alveolar bone formation and PDL remodeling.