The biotransformation of oleuropein in rats
The biotransformation of oleuropein in rats
复制标题
橄榄苦苷在大鼠体内的生物转化
DOI：
10.1002/bmc.2922
复制
发表时间：
2013-09-01
影响因子：
1.8
通讯作者：
Qian, Tianxiu
中科院分区：
文献类型：
--
作者：
Lin, Peiyan;Qian, Weidong;Qian, Tianxiu
建立了一种高灵敏度、高特异性的LC-MS/MS方法，研究橄榄苦苷在大鼠体内的生物转化。采用电喷雾负离子电离液相色谱-串联质谱法对口服给药后的大鼠粪便和尿液进行测定。测定过程包括用乙酸乙酯从大鼠粪便和尿液中提取母本橄榄苦苷及其代谢物。色谱分离采用0.1%甲酸水溶液和甲醇，梯度程序，流速0.50 mL/min， RP-C-18柱，总运行时间31 min。该方法可用于大鼠粪便和尿液中橄榄苦苷及其代谢物的同时测定。去糖基化、水解、氧合和甲基化是橄榄苦苷在大鼠胃肠道中的主要代谢途径，三种代谢产物在口服后24 h内被血液循环系统吸收。版权所有：John Wiley & Sons， Ltd
A highly sensitive and specific LC-MS/MS method was developed to investigate the in vivo bio-transformation of oleuropein in rat. Rat feces and urine samples collected after oral administration were determined by liquid chromatography coupled to tandem mass spectrometry with electrospray ionization in the negative-ion mode. The assay procedure involves a simple liquid-liquid extraction of parent oleuropein and the metabolite from rat feces and urine with ethyl acetate. Chromatographic separation was operated with 0.1% formic acid aqueous and methanol in gradient program at a flow rate of 0.50 mL/min on an RP-C-18 column with a total run time of 31 min. This method was successfully applied to simultaneous determination of oleuropein and its metabolites in rat feces and urine. De-glucosylation, hydrolysis, oxygenation and methylation were found to comprise the major metabolic pathway of oleuropein in rat gastrointestinal tract and three metabolites were absorbed into the blood circulatory system within 24 h after oral administration. Copyright (C) 2013 John Wiley & Sons, Ltd.