Clinical significance of combination study of apoptotic factors and proliferating cell nuclear antigen in estimating the prognosis of hepatocellular carcinoma
Clinical significance of combination study of apoptotic factors and proliferating cell nuclear antigen in estimating the prognosis of hepatocellular carcinoma
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DOI：
10.1002/jso.20006
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发表时间：
2004-01-01
影响因子：
2.5
通讯作者：
Kuno, T
中科院分区：
文献类型：
--
作者：
Osada, S;Saji, S;Kuno, T
背景与目的：肝细胞癌（HCC）是最常见的复发性疾病之一，影响患者的预后。方法：对153例原发性肝癌进行增殖细胞核抗原（PCNA）、p53、Bax免疫组化检测。分析了这些因素与组织学分级、肝内转移（IM）、肿瘤大小、血清甲胎蛋白（AFP）水平及预后的关系。结果：PCNALI在胃癌中明显增高，且与肿瘤大小相关。PCNALI和肿瘤直径本身可作为患者预后的良好预测指标，两者联合应用更能反映患者的预后。p53阳性者AFP值明显增高。p53的表达与组织学类型有关。在Bax阴性的主要肿瘤中发现IM的存在。Bax的表达与组织学类型无关。p53和Bax的表达可作为判断低级别HCC患者预后的指标，而常规病理学检查不能发现两者的差异。结论：结合免疫组化结果和临床特征可作为判断HCC预后的重要辅助手段。(C)2003 Wiley-Liss，Inc.
Background and Objectives: Hepatocellular carcinoma (HCC) is one of the most common recurrence diseases, which affects the patient's prognosis. The aim of this report is to evaluate recurrence risk after primary treatment by the combination study with the clinical features and immunohistological findings.Methods: 153 removable HCCs were examined by inummohistochemical study of the proliferating cell nuclear antigen (PCNA), p53, or Bax. The relationships of these factors with histological grades, the presence of intra-hepatic metastasis (IM), tumor size, value of serum alpha-fetoprotein (AFP), and prognosis were studied. PCNA labeling index (LI) was calculated to count positive nuclei in 1,000 cells.Results: PCNALI was significantly higher in cancer and correlated with tumor size. PCNALI and the tumor diameter in themselves could be a good predictor for patient prognosis and the combination study of them was an even stronger indicator. The value of AFP was significantly higher in positive p53 cases. The incidence of p53 was associated with histological types. The presence of IM was found in negative Bax cases of main tumors. The appearance of Bax was not correlated with histological types. The incidence of p53 or Bax was indicated to distinguish the patient prognosis of the lower grade histological cases, in which differences could not be found by the routine histological study.Conclusions: The combination study of the immunohistochemical findings and the clinical features could be one of the most important aids in interpreting the status of HCC. (C) 2003 Wiley-Liss, Inc.