Multimodal confocal microscopy for diagnosing nonmelanoma skin cancers
Multimodal confocal microscopy for diagnosing nonmelanoma skin cancers
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DOI：
10.1002/lsm.20578
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发表时间：
2007-10-01
影响因子：
2.4
通讯作者：
Yaroslavsky, Anna N.
中科院分区：
文献类型：
--
作者：
Al-Arashi, Munir Y.;Salomatina, Elena;Yaroslavsky, Anna N.
背景和意义：皮肤癌的标准诊断程序是侵入性活检，然后进行组织病理学评估。活检可能会导致疤痕和感染。一种可靠的、高分辨率、高对比度的可疑性病变的非侵入性成像方法将是有价值的。在这项研究中，评价了染料增强多模式共聚焦显微镜检测非黑色素瘤皮肤癌的适用性。材料和方法：实验使用新鲜肿瘤材料，分别用0.2 mg/ml和0.05 mg/ml亚甲基蓝(MB)或甲苯胺蓝(TB)水溶液染色。分别用656 nm和633 nm的光激发MB和TB染色的皮肤样品的反射、荧光和荧光偏振图像。荧光发射和各向异性在690~710 nm之间。此外，还获得了830 nm处的参考反射率图像。总共，我们对每种肿瘤类型的至少10个样本进行成像、分析和组织学比较，包括结节状基底细胞癌(BCC)、浸润性基底细胞癌和鳞状细胞癌(SCC)。结果与结论：在所有研究的肿瘤类型中，荧光图像中皮肤结构的形态特征和外观与相应的组织学有很好的相关性。多光谱反射图像提供关于组织光谱响应的信息，并且是对荧光图像的补充。荧光偏振检测到的癌组织和正常组织的差异可用于癌组织的鉴别。我们的结果表明，使用多模式共聚焦显微镜作为实时检测皮肤病理的工具是可行的。
Background and Significance: The standard diagnostic procedure for skin cancers is invasive biopsy followed by histopathological evaluation. The biopsy may result in scarring and infection. A reliable way to noninvasively image suspicious lesions with high resolution and contrast would be valuable. In this study, the suitability of dye-enhanced multimodal confocal microscopy for the detection of nonmelanoma skin cancers was evaluated. Materials and Methods: For the experiments we used fresh tumor material stained using 0.2 mg/ml or 0.05 mg/ml aqueous solutions of methylene blue (MB) or toluidine blue TB), respectively. Reflectance, fluorescence, and fluorescence polarization images of skin specimens stained with MB and TB were excited by 656 nm and 633 nm light, respectively. Fluorescence emission and anisotropy were registered between 690 nm and 710 nm. In addition, reference reflectance images at 830 nm were acquired. In total we imaged, analyzed, and compared to histology at least 10 samples of each tumor-type including nodular basal cell carcinoma (BCC), infiltrative basal cell carcinoma, and squamous cell carcinoma (SCC). Results and Conclusion: The morphological features and appearance of skin structures in the fluorescence images correlate well with corresponding histology for all investigated tumor-types. Multi-spectral reflectance images provide information on the tissue spectral responses and are complimentary to the fluorescence images. The differences detected by fluorescence polarization in cancerous and normal structures may be used for cancerous tissue discrimination. Our results indicate the feasibility of using multimodal confocal microscopy as real-time tool for detecting skin pathology.