Differential effects of recombinant human interleukin‐13 on the in vitro growth of human haemopoietic progenitor cells
Differential effects of recombinant human interleukin‐13 on the in vitro growth of human haemopoietic progenitor cells
复制标题
重组人白介素-13对人造血祖细胞体外生长的不同影响
DOI：
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发表时间：
1995
影响因子：
6.5
通讯作者：
Z. Han
中科院分区：
文献类型：
--
作者：
X. Xi;N. Schlegel;J. Caen;A. Minty;S. Fournier;D. Caput;P. Ferrara;Z. Han
摘要研究了重组人白细胞介素（IL）-13对人脐带血（CB）非贴壁单核细胞（NAMC）或高富集CD 34+细胞体外造血的影响。IL-13以剂量依赖性方式显著增加了在补充再生障碍性贫血血清（AAS）和植物血红蛋白刺激的人外周血白细胞条件培养基（PHA-LCM）的血浆凝块系统中培养的NAMC或CD 34+细胞的巨核细胞（MK）集落形成。使用改良的血浆凝块培养物的实验，其中加入正常AB血清和各种细胞因子以代替AAS和PHA-LCM，证明在IL-13存在下，尤其是与IL-3组合时，MK菌落数增加。然而，IL-13对两种血浆凝块培养物中的粒细胞-巨噬细胞（GM）集落形成无刺激作用，但在某些情况下有轻微抑制作用。此外，在存在IL-3、GM-CSF、Epo、G-CSF或其组合的情况下，GM祖细胞在甲基纤维素培养系统中的生长被IL-13的添加显著抑制。另一方面，需要高浓度（100 ng/ml）的IL-13以在相同的甲基纤维素培养物下引起对BFU-E衍生的集落的生长的轻微抑制。这些结果表明，IL-13单独和与IL-3的作用协同促进MK集落形成，但它抑制GM和红系祖细胞的体外生长。
Summary. Effects of recombinant human interleukin (IL)‐13 on in vitro haemopoiesis from non‐adherent mononuclear cells (NAMC) or highly enriched CD34+ cells of human cord blood (CB) were studied. IL‐13 significantly increased megakaryocyte (MK) colony formation from either NAMC or CD34+ cells cultured in a plasma clot system supplemented with aplastic anaemia serum (AAS) and phytohaemag‐glutinin‐stimulated human peripheral blood leucocyte‐conditioned medium (PHA‐LCM) in a dose‐dependent manner. Experiments using a modified plasma clot culture, in which normal AB serum and various cytokines were added to replace AAS and PHA‐LCM, demonstrated an increased MK colony number in the presence of IL‐13, especially in combination with IL‐3. However, IL‐13 had no stimulatory effect, but rather a slight inhibitory effect in some cases on granulocyte‐macrophage (GM) colony formation in both plasma clot cultures. Furthermore, the growth of GM progenitor cells in a methylcellulose culture system in the presence of IL‐3, GM‐CSF, Epo, G‐CSF or in combination was significantly inhibited by the addition of IL‐13. On the other hand, high concentrations (lOOng/ml) of IL‐13 were needed to cause a slight inhibition on the growth of BFU‐E‐derived colonies under the same methylcellulose culture. These results indicate that IL‐13, alone and synergistically with the effect of IL‐3, promotes MK colony formation, but it inhibits the growth of GM and erythroid progenitor cells in vitro.
乙酰水杨酸刺激小鼠巨核细胞前体细胞。
DOI：
10.3181/00379727-194-43081
发表时间：
1990
期刊：
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)
影响因子：
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作者：
Sullivan,PS;McDonald,TP
通讯作者：
McDonald,TP
人脐带血中糖蛋白 IIb/IIIa 阳性细胞的表征：它们作为巨核细胞祖细胞的潜在用途。
影响因子：
20.3
作者：
Zucker-Franklin,D;Yang,JS;Grusky,G
通讯作者：
Grusky,G