The p53/p21 DNA damage-signaling pathway is defective in most meningioma cells
The p53/p21 DNA damage-signaling pathway is defective in most meningioma cells
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DOI：
10.1007/s11060-006-9301-3
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发表时间：
2007-05-01
影响因子：
3.9
通讯作者：
Aboussekhra, Abdelilah
中科院分区：
文献类型：
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作者：
Al-Khalaf, Huda H.;Lach, Boleslaw;Aboussekhra, Abdelilah
虽然脑膜瘤是中枢神经系统最常见的一类肿瘤，但其发生和发展的分子机制仍不清楚。本研究应用免疫印迹技术研究了抑癌蛋白p53、p21和PTEN在脑膜瘤细胞中的表达水平。我们还研究了p21和p53对紫外线和γ射线的诱导作用。我们目前的证据表明，p53/p21依赖的γ-射线信号通路是有缺陷的，在这些细胞的8个（62%）。此外，我们已经表明，肿瘤抑制因子p21，p53和PTEN蛋白在这些细胞中的表达不同，每种蛋白的最低和最高水平之间的差异高达40倍。提示肿瘤抑制因子p53/p21信号通路和PTEN在良性脑膜瘤的发生发展中起重要作用。
Although meningiomas represent the most common class of tumors of the central nervous system, the molecular events underlying their genesis and development are still not well defined. In the present study we have used the immuno-blotting technique to study the expression level of the tumor suppressor proteins p53, p21 and PTEN in primary meningioma cells. We have also studied the induction of p21 and p53 in response to both UV light and gamma-rays. We present evidence that the p53/p21-dependent gamma-ray signaling pathway is defective in 5 out of 8 (62%) of these cells. Furthermore, we have shown that the tumor suppressor p21, p53 and PTEN proteins are differently expressed in these cells, with up to 40-folds difference between the lowest and the highest levels of each protein. These results suggest that the tumor suppressors p53/p21 signaling pathway and PTEN play important roles in the development of benign meningiomas.