Identification of core genes in prefrontal cortex and hippocampus of Alzheimer's disease based on mRNA-miRNA network.
Identification of core genes in prefrontal cortex and hippocampus of Alzheimer's disease based on mRNA-miRNA network.
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基于mRNA-miRNA网络识别阿尔茨海默病前额皮质和海马核心基因
DOI：
10.1111/jcmm.17593
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发表时间：
2022-12
影响因子：
5.3
通讯作者：
中科院分区：
文献类型：
--
作者：
阿尔茨海默病（AD）是一种神经退行性疾病，伴有认知障碍和异常心理行为。目前尚无有效的治疗方法。早期诊断标志物的开发和潜在治疗靶点的挖掘是重要策略之一。本研究旨在探索海马体和前额叶皮层（与 AD 高度相关的两个大脑区域）中与 AD 相关的潜在生物标志物或治疗靶点。 AD 患者和健康对照之间的差异表达基因和 miRNA 是从 Gene Expression Omnibus 数据库中获得的。构建了 mRNA-miRNA 网络，通过蛋白质-蛋白质相互作用分析筛选出参与 AD 的关键基因，随后通过独立数据集和 AD 小鼠模型中的 qPCR 进行验证。我们的研究结果表明，包括 CALN1、TRPM7、ATR、SOCS3、MOB3A 和 OGDH 在内的 6 个枢纽基因被认为与 AD 的发病机制有关。 Western blot分析进一步确定CALN1、ATR和OGDH是AD可能的生物标志物和治疗靶点。此外，6种可能的miRNA生物标志物也已通过qPCR在AD动物模型上得到验证。我们的研究结果可能有助于 AD 的临床诊断和早期预防。
Alzheimer's disease (AD) is a neurodegenerative disorder with cognitive impairment and abnormal mental behaviour. There is currently no effective cure. The development of early diagnostic markers and the mining of potential therapeutic targets are one of the important strategies. This study aimed to explore potential biomarkers or therapeutic targets related to AD in the hippocampus and prefrontal cortex, two brain regions highly related to AD. Differentially expressed genes and miRNAs between AD patients and healthy controls were obtained from the Gene Expression Omnibus database. The mRNA‐miRNA network was constructed and key genes involved in AD were screened out by protein–protein interaction analysis, and were subsequently verified by independent datasets and qPCR in an AD mouse model. Our findings showed that six hub genes including CALN1, TRPM7, ATR, SOCS3, MOB3A and OGDH were believed to be involved in the pathogenesis of AD. Western blot analysis further determined that CALN1, ATR and OGDH were the possible biomarkers and therapeutic targets for AD. In addition, 6 possible miRNAs biomarkers have also been verified by qPCR on AD animal models. Our findings may benefit clinical diagnosis and early prevention of AD.
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影响因子：
15.1
作者：
Brinkmalm A;Brinkmalm G;Honer WG;Frölich L;Hausner L;Minthon L;Hansson O;Wallin A;Zetterberg H;Blennow K;Öhrfelt A
通讯作者：
Öhrfelt A
抑制 miR-331-3p 和 miR-9-5p 可改善阿尔茨海默病
影响因子：
12.4
作者：
Chen ML;Hong CG;Yue T;Li HM;Duan R;Hu WB;Cao J;Wang ZX;Chen CY;Hu XK;Wu B;Liu HM;Tan YJ;Liu JH;Luo ZW;Zhang Y;Rao SS;Luo MJ;Yin H;Wang YY;Xia K;Tang SY;Xie H;Liu ZZ
通讯作者：
Liu ZZ
影响因子：
4.2
作者：
Gullett JM;Chen Z;O'Shea A;Akbar M;Bian J;Rani A;Porges EC;Foster TC;Woods AJ;Modave F;Cohen RA
通讯作者：
Cohen RA
影响因子：
6.1
作者：
Hebert, Sebastien S.;Horre, Katrien;De Strooper, Bart
通讯作者：
De Strooper, Bart
影响因子：
3.6
作者：
Gandbhir, Omkar;Sundaram, Pazhani
通讯作者：
Sundaram, Pazhani