Molecular Characterization of Cryptosporidium Isolates from Calves in Ishikari District, Hokkaido, Japan
Molecular Characterization of Cryptosporidium Isolates from Calves in Ishikari District, Hokkaido, Japan
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DOI：
10.1292/jvms.12-0435
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发表时间：
2013-07-01
影响因子：
1.2
通讯作者：
Kato, Kentaro
中科院分区：
文献类型：
--
作者：
Murakoshi, Fumi;Tozawa, Yoriko;Kato, Kentaro
牛是隐孢子虫的主要宿主。新生小牛的隐孢子虫病与生长迟缓、体重减轻和小牛死亡以及人类的人畜共患感染有关。从日本北海道石狩区的小牛中采集粪便样品，并通过PCR和序列分析进行检查。在2012年5 - 6月采集的107份粪便样本中，25份（23%）隐孢子虫阳性，其中8份（7%）隐孢子虫阳性。parvum，10株（9%）有C.牛型7例（7%）; ryanae这是第一次C。在北海道发现了ryanae。此外，这也是C. ryanae的断奶前小牛在日本。显微镜下漂浮法是筛选隐孢子虫种的传统方法，但有时会导致隐孢子虫检出率低，卵囊脱落强度低。如果使用分子诊断工具检查有或没有腹泻的小牛，C。bovis和C.在日本其他地区包括北海道也可能检测到ryanae。在这里，人畜共患病的物种，C。也观察到了小孢子虫。因此，小牛可能是人类和其他动物隐孢子虫感染的潜在来源。C.细小病毒与犊牛腹泻有统计学相关性。
Cattle are major hosts of Cryptosporidium. Cryptosporidiosis in neonatal calves is associated with retarded growth, weight loss and calf mortality, and zoonotic infections in humans. Fecal samples were collected from calves in Ishikari District, Hokkaido, Japan and examined by PCR and sequence analyses. Among the 107 fecal samples collected in May and June 2012,25 (23%) were positive for Cryptosporidium, including 8 samples (7%) having C. parvum, 10 (9%) having C. bovis and 7 (7%) having C. ryanae. This is first time C. ryanae has been detected in Hokkaido. Furthermore, it is the first detection of C. ryanae from pre-weaned calves in Japan. Microscopic observation with the flotation method is powerful and traditional tool for screening for Cryptosporidium species, but it sometimes leads to low detection of Cryptosporidium with low oocyst shedding intensity. If calves with or without diarrhea are examined using the molecular diagnostic tools, C. bovis and C. ryanae might be detected in other areas of Japan including Hokkaido. Here, the zoonotic species, C. parvum, was also observed. Therefore, calves can be potential sources of cryptosporidial infections for humans and other animals. The detection of C. parvum was statistically correlated with diarrhea in calves.