Enhanced Osteogenic Activity of TiO2 Nanorod Films with Microscaled Distribution of Zn-CaP
Enhanced Osteogenic Activity of TiO2 Nanorod Films with Microscaled Distribution of Zn-CaP
复制标题
Zn-CaP 微尺度分布增强 TiO2 纳米棒薄膜的成骨活性
DOI：
10.1021/acsami.6b01284
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发表时间：
2016-03-23
影响因子：
9.5
通讯作者：
Wang, Huiming
中科院分区：
文献类型：
--
作者：
He, Meng;Chen, Xiaoyi;Wang, Huiming
微/纳米级的形貌和材料表面的生物活性成分被认为在它们与细胞的相互作用中起着至关重要的作用。然而，用可生物降解的组合物进一步修饰特殊形貌仍然是一个挑战，反之亦然。在这项研究中，二氧化钛纳米棒薄膜覆盖的微米级分布的含锌磷酸钙（Zn-CaP），试图创建一个微/纳米级的形貌和Zn 2+释放能力。MC 3 T3-E1细胞在Zn-CaP分布稀疏的TiO 2纳米棒膜（TiO 2/S-ZCP）上培养的生物学反应显著高于Zn-CaP分布密集的TiO 2纳米棒膜（TiO 2/D-ZCP）和Zn-CaP完全覆盖的TiO 2纳米棒膜（F-ZCP）。基于ALP、相关基因表达和细胞外基质矿化的评估，证明TiO 2/S-ZCP膜比F-ZCP和TiO 2/D-ZCP膜更强地促进成骨分化。TiO 2/S-ZCP膜上的成骨分化能力较强，这是由于Zn-CaP膜表面形成的微/纳米级形貌促进了细胞的粘附和丝状伪足的延伸，并在初始阶段诱导了细胞的分化取向。Zn ~（2+）的释放促进了细胞的分化。因此，我们认为更好。微/纳米形貌的组织化和生物活性离子在表面上的释放将是增强矫形和牙科植入物的成骨活性的有希望的方式。
The topography at the micro-/nanoscale level and bioactive composition of material surfaces have been thought to play vital roles in their interactions with cells. However, it is still a challenge to further modify special topography with biodegradable composition or vice versa. In this study, TiO2 nanorod films covered with microscale-distributed Zn-containing calcium phosphate (Zn-CaP) were prepared, trying to create a micro-/nanoscale topography and Zn2+ release capability. MC3T3-E1 cells cultured on TiO2 nanorod film with sparsely distributed Zn-CaP (TiO2/S-ZCP) had significantly higher biological responses than those on the films with densely distributed Zn-CaP (TiO2/D-ZCP) and fully covered Zn-CaP (F-ZCP). TiO2/S-ZCP film was demonstrated to facilitate osteogenic differentiation much more strongly than F-ZCP and TiO2/D-ZCP films based on evaluations of ALP, related gene expressions, and extracellular matrix mineralization. The higher osteogenic differentiation on TiO2/S-ZCP film is ascribed to the micro-/nanoscale topography from Zn-CaP coverage promoting cell adhesion and filopodia extension, and inducing differentiation-orientation in the initial stage. And consequently Zn2+ release results in enhancement of differentiation. Therefore, we believe that better. organization of the micro-/nanotopography and bioactive ion release on the surface would be a promising way to enhance osteogenic activity for orthopedic and dental implants.