Identification of a novel splice variant of heat shock cognate protein 70 after chronic antidepressant treatment in rat frontal cortex.
Identification of a novel splice variant of heat shock cognate protein 70 after chronic antidepressant treatment in rat frontal cortex.
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大鼠额叶皮层长期抗抑郁治疗后热休克同源蛋白 70 的新型剪接变体的鉴定。
DOI：
10.1006/bbrc.1999.1067
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发表时间：
1999
影响因子：
3.1
通讯作者：
Teruhiko Higuchi
中科院分区：
文献类型：
--
作者：
M. Yamada;M. Yamada;Yuji Kiuchi;Keinosuke Nara;Yuko Kanda;Shigeru Morinobu;Kazutaka Momose;K. Oguchi;K. Kamijima;Teruhiko Higuchi
在这项研究中，我们鉴定了 70 kDa 热休克同源蛋白 (HSC70) 的一种新型剪接变体，同时筛选了长期抗抑郁治疗后大鼠大脑中差异表达的分子。该克隆被命名为 HSC49，缺乏大鼠 HSC70 的 470 bp 核苷酸。 HSC49编码442个氨基酸残基，计算分子量为48.6 kDa。 DNA序列分析显示HSC49缺乏HSC70基因的整个外显子7和外显子8。与对照组相比，长期使用抗抑郁药丙咪嗪或舍曲林治疗，可使大鼠额叶皮层的 mRNA 水平分别增加 38.5% 或 22.5%。 Western blot分析还显示，抗抑郁治疗后HSC49的蛋白表达增加。我们的数据表明 HSC49 可能是长期抗抑郁治疗后诱导的常见分子之一。
In this study, we identified a novel splice variant of 70-kDa heat shock cognate protein (HSC70), while screening differentially expressed molecules in rat brain after chronic antidepressant treatment. This clone, named HSC49, lacked 470 bp of nucleotides of rat HSC70. HSC49 encoded 442 amino acid residues with a calculated molecular mass of 48.6 kDa. DNA sequence analysis revealed that HSC49 lacked the entire Exon 7 and Exon 8 of the HSC70 gene. Chronic treatment with antidepressant, imipramine or sertraline, induced a 38.5 or 22.5% increase in mRNA levels in rat frontal cortex, respectively, when compared to controls. Western blot analysis also revealed that the protein expression of HSC49 was increased after antidepressant treatment. Our data suggest that HSC49 may be one of the common molecules induced after chronic antidepressant treatment.
肾上腺和主动脉热休克蛋白 70 表达的多巴胺能调节。
影响因子：
4.8
作者：
Blake,MJ;Buckley,DJ;Buckley,AR
通讯作者：
Buckley,AR