In vivo gene transfection with hepatocyte growth factor via the pulmonary artery induces angiogenesis in the rat lung
In vivo gene transfection with hepatocyte growth factor via the pulmonary artery induces angiogenesis in the rat lung
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DOI：
10.1161/cir.0000032879.55215.f4
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发表时间：
2002-09-24
期刊：
影响因子：
37.8
通讯作者：
Matsuda, H
中科院分区：
文献类型：
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作者：
Ono, M;Sawa, Y;Matsuda, H
背景-最近的研究表明，肝细胞生长因子（HGF）的基因转移诱导冠状动脉和外周动脉疾病的血管生成。我们调查的能力，基因转移与人肝细胞生长因子诱导血管生成的大鼠lung.Methods和结果的左肺选择性转染的cDNA编码人肝细胞生长因子通过左肺动脉，使用HVJ-脂质体方法（H组）;大鼠转染相同的载体缺乏肝细胞生长因子基因作为对照（C组）。与C组相比，HGF基因转染组在转染后7天显著增加了左肺毛细血管密度（15.0+/-1.3vs8.0 +/-1.7 mm（2），P
Background-Recent studies have demonstrated that gene transfer with hepatocyte growth factor (HGF) induces angiogenesis for coronary and peripheral artery diseases. We investigated the ability of gene transfer with human HGF to induce angiogenesis in the rat lung.Methods and Results-The left lung was selectively transfected with a cDNA encoding human HGF via the left pulmonary artery, using the HVJ-liposome method (H group); rats transfected with the same vector lacking the HGF gene served as controls (C group). HGF gene transfer significantly increased the capillary density in the left lung compared with the C group 7 days after transfection (15.0+/-1.3 versus 8.0+/-1.7 mm(2), P