ENDOGENOUS TRANSCRIPTION OCCURS AT THE 1-CELL STAGE IN THE MOUSE EMBRYO
ENDOGENOUS TRANSCRIPTION OCCURS AT THE 1-CELL STAGE IN THE MOUSE EMBRYO
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DOI：
10.1006/excr.1995.1130
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发表时间：
1995-05-01
影响因子：
3.7
通讯作者：
DEBEY, P
中科院分区：
文献类型：
--
作者：
BOUNIOL, C;NGUYEN, E;DEBEY, P
在大多数动物物种中，胚胎发生的最早阶段是由母系遗传成分调节的，至少直到合子基因组的激活为止。尽管很久以前在小鼠的2细胞阶段就检测到了源自胚胎基因组激活的第一个蛋白质，但受精后转录活性恢复的确切时间仍然存在争议。关于这一关键事件的任何新信息都与胚胎转基因和克隆的实践以及受精后核过程调节的一般理解相关。使用允许检测体内 RNA 合成的新荧光方法，我们表明 RNA 聚合酶 II 的内源转录早在 1 细胞阶段就明确发生。此外，我们证明转录首先在父本原核中启动。 (C) 1995 学术出版社
In most animal species, the earliest stages of embryogenesis are regulated by maternally inherited components, at least until the activation of the zygotic genome, Although the first proteins derived from the activation of the embryonic genome have been detected long ago at the 2-cell stage in the mouse, the exact timing of transcriptional activity resumption after fertilization is still a matter of debate. Any new information about this critical event is relevant to the practice of transgenesis and cloning of embryos as well as to the general understanding of the regulation of nuclear processes following fertilization. Using a new fluorescent method allowing the detection of in vivo RNA synthesis, we show that endogenous transcription by RNA polymerase II takes place unambiguously as early as at the late 1-cell stage, Furthermore, we demonstrate that transcription is first initiated in the paternal pronucleus. (C) 1995 Academic Press, Inc.