Resistance against beet armyworms and cotton aphids in caffeine-producing transgenic chrysanthemum
Resistance against beet armyworms and cotton aphids in caffeine-producing transgenic chrysanthemum
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DOI：
10.5511/plantbiotechnology.11.0510a
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发表时间：
2011-01-01
影响因子：
1.6
通讯作者：
Sano, Hiroshi
中科院分区：
文献类型：
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作者：
Kim, Yun-Soo;Lim, Soon;Sano, Hiroshi
构建了同时表达三种参与咖啡因生物合成途径的n -甲基转移酶的转基因菊花植株。由此产生的植物在大约3 μ g（-1）新鲜组织中产生咖啡因，并对食草动物进行了驱避测试。当饥饿的甜菜夜蛾（Spodoptera exigu）二龄毛虫被允许进食时，它们吃下了野生型植物4.4毫米(2)的叶盘，而转基因植物的叶盘不到1.5毫米(2)。对三龄棉蚜（Aphis gossypii）进行选择试验，27只聚集在野生型叶片上，6只聚集在转基因叶片上。这些结果表明，产咖啡因菊对食草动物、鳞翅目毛虫和蚜虫具有抗性，这两种害虫都是农业中最严重的害虫之一。我们建议，该方法可以实际应用于各种重要的植物物种赋予抵抗生物胁迫。
Transgenic chrysanthemum plants were constructed to simultaneously express three N-methyltransferases involved in caffeine biosynthetic pathways. Resulting plants produced caffeine at approximately 3 mu g g(-1) fresh tissue, and were tested for herbivore repellence. When starved second-instar caterpillars of beet armyworms (Spodoptera exigu) were allowed to feed, they ate up to 4.4 mm(2) of leaf discs from the wild type plants, while less than 1.5 mm(2) of those from the transgenic plants. When third-instars of cotton aphid (Aphis gossypii) were subjected to a choice-test, 27 gathered on wild type leaves, and 6 on transgenic leaves. These results indicate that caffeine-producing chrysanthemum is resistant against herbivores, lepidoptera caterpillars and aphids, both being one of the most serious pests in agriculture. We propose that the method can be practically applied to a variety of important plant species to confer resistance against biotic stresses.